Recombinant Mouse Mesoderm posterior protein 2 (Mesp2)

Code CSB-YP013710MO
MSDS
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Source Yeast
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Code CSB-EP013710MO
MSDS
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Source E.coli
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Code CSB-EP013710MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013710MO
MSDS
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Source Baculovirus
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Code CSB-MP013710MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mesp2
Uniprot No.
Alternative Names
Mesp2; Mesoderm posterior protein 2
Species
Mus musculus (Mouse)
Expression Region
1-370
Target Protein Sequence
MAQSSPPQSL QGLVPLGLLP GLGLGSAIGL HVSGLVLRFV RFLPFYATRR PSQPAGPARS TRTTQATAPR RTRPAPAGGQ RQSASEREKL RMRTLARALQ ELRRFLPPSV APAGQSLTKI ETLRLAIRYI GHLSALLGLS EDSLRRRRRR SADAAFSHRC PQCPDGGSPS QAQMLGPSLG SAMSSGVSWG CPPACPGPLI SPENLGNRIS NVDPRVTPPY CPQIQSPLHQ SLERAADSSP WAPPQACPGM QMSPEPRNKT GHWTQSTEPA ELTKVYQSLS VSPEPRLSLG SPLLLPRPSC QRLQPQPQPQ PQWGCWGHDA EVLSTSEDQG SSPALQLPVA SPTPSSGLQL SGCPELWQED LEGPPLNIFY
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. May play a role with Tcf15 in the differentiation of myotomal and sclerotomal cells by regulating Pax family genes. Controls also the expression of the protocadherin PCDH8/PAPC, EPHA4, RIPPLY2, NOTCH2, FGFR1, and CER1. Binds to the E-boxes within the EPH4A and RIPPLY2 enhancers.
Gene References into Functions
  1. conclusion was supported by analyses of Mesp2 KO and Ripply1/2 double KO embryos lacking rostral and caudal properties, respectively PMID: 23727513
  2. current observations of the spatiotemporal disorder of vertebral organogenesis in the Mesp2-null mice provide further insight into the pathogenesis of SCDO and STDO, and the physiological development of the axial skeleton PMID: 23238123
  3. Data demonstrate that Mesp2 is a novel component involved in the suppression of Notch target genes. PMID: 21098559
  4. Data propose a novel function of Notch signaling, in which a progressive oscillating wave of Notch activity is translated into the rostral-caudal polarity of a somite by regulating Mesp2 expression in the anterior presomitic mesoderm. PMID: 20335362
  5. A bHLH-type transcription factor, Mesp2, plays an essential role in somite segmentation in mice. PMID: 11973278
  6. Data describe the genetic interactions between Dll1, Dll3, Mesp2 and Psen1, and the roles of Dll1- and Dll3-Notch pathways, with or without Psen1, in rostrocaudal patterning. PMID: 12900443
  7. Mesp2 is responsible for the rostro-caudal patterning process itself in the anterior presomitic mesoderm, via cellular interaction. PMID: 15677726
  8. developmental protein "wavefront" is generated by suppression of Notch activity by mesoderm posterior 2 (Mesp2) through induction of the lunatic fringe gene (Lfng) PMID: 15902259
  9. Tbx6 directly binds to the Mesp2 gene upstream region and mediates Notch signaling, and subsequent Mesp2 transcription, in the anterior presomitic mesoderm. PMID: 16505380
  10. Forced expression of Mesp2 in somitic cells results in the activation of Epha4. PMID: 16728472
  11. Ripply2-Mesp2 negative-feedback loop is essential for the periodic generation of the rostro-caudal polarity within a somite. PMID: 17360776
  12. patterning events by basic helix-loop-helix-type transcription factors have deep impacts on regional chondrogenic and myogenic differentiation of somitic cells PMID: 17477400
  13. Mesp2 protein leads to the suppression of Tbx6 protein expression post-translationally via rapid degradation mediated by the ubiquitin-proteasome pathway PMID: 18579680
  14. An Mesp2 enhancer knockout mouse bearing mutations in two crucial Tbx6 binding sites does not express Mesp2 in the presomitic mesoderm. PMID: 18849530

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Subcellular Location
Nucleus.
Database Links

KEGG: mmu:17293

UniGene: Mm.31472

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