Recombinant Mouse Nuclear factor NF-kappa-B p100 subunit (Nfkb2), partial

Code CSB-YP896226MO
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Source Yeast
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Code CSB-EP896226MO
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Source E.coli
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Code CSB-EP896226MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896226MO
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Source Baculovirus
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Code CSB-MP896226MO
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Nfkb2; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2) [Cleaved into: Nuclear factor NF-kappa-B p52 subunit]
Mus musculus (Mouse)
Protein Length
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
Gene References into Functions
  1. in vivo bioluminescence imaging in a transgenic model of inflammation showed that luciferase activity coincided with endogenous p52/p100 Nfkb2 expression PMID: 29680659
  2. our studies for the first time establish p100 as a key tumor suppressor of bladder cancer growth PMID: 27095572
  3. hyperresponsive promoter engaged the RelA:p52 dimer generated during costimulation of macrophages through TLR4 and LTbetaR to trigger synthesis of IkappaBalpha at late time points, which prevented the late-acting RelA cross-talk response. PMID: 27923915
  4. results suggest that changes in the relative concentrations of RelB, NIK:IKK1, and p100 during noncanonical signaling modulate this transitional complex and are critical for maintaining the fine balance between the processing and protection of p100. PMID: 27678221
  5. Results suggest that the RelB/NF-kappaB2 pathway regulates T cell migration to autoimmune targets in Sjogren syndrome through TGFbeta/TGFbetaR-dependent regulation of CXCL12/CXCR4 signaling. PMID: 28804991
  6. MKK4 activates non-canonical NFkappaB signaling by promoting NFkappaB2-p100 processing. PMID: 28733031
  7. the aberrant proliferative capacity of Brca1(-/-) luminal progenitor cells is linked to the replication-associated DNA damage response, where proliferation of mammary progenitors is perpetuated by damage-induced, autologous NF-kappaB signaling. PMID: 27292187
  8. RelB is processed by CO2 in a manner dependent on a key C-terminal domain located in its transactivation domain. Loss of the RelB transactivation domain alters NF-kappaB-dependent transcriptional activity, and loss of p100 alters sensitivity of RelB to CO2 PMID: 28507099
  9. we identify in this study a critical role for the combined activity of the RELB and NF-kappaB2 subunits in B cell homeostasis that cannot be compensated for by the canonical NF-kappaB pathway under physiological conditions. PMID: 26851215
  10. Mechanistically the LncRNA-HIT siRNA treatments impacted pro-chondrogenic gene expression by reducing H3K27ac or p100 activity, confirming that LncRNA-HIT is essential for chondrogenic differentiation in the limb mesenchyme PMID: 26633036
  11. Nfkb2 connected lymphotoxin signal within the intestinal niche in reinforcing epithelial innate inflammatory RelA/NF-kappaB response to Citrobacter rodentium infection, while Nfkb2(-/-) mice succumbed to gut infections owing to stromal defects. PMID: 25905673
  12. Data indicate a coupled increase in the expression of renal, relA, NF-kB2, and p53 genes and proteins during folic acid induced acute kidney injury (AKI). PMID: 25559736
  13. Loss of c-REL but not NF-kappaB2 prevents autoimmune disease driven by FasL mutation. PMID: 25361085
  14. NF-kappaB2/p52 is necessary for the development of colitis, whilst c-Rel-mediated signalling regulates colonic epithelial cell turnover following DNA damage. PMID: 25727407
  15. Nuclear translocation of c-Rel was enhanced in p100-deficient cells. p100, and not the processed p52 form, associated with c-Rel in the steady state and dissociated immediately after lipopolysaccharide stimulation in wild-type dendritic cells PMID: 25305492
  16. The unique ability of p100/IkappaBdelta to stably interact with all NF-kappaB subunits by forming kappaBsomes demonstrates its importance in sequestering NF-kappaB subunits and releasing them as dictated by specific stimuli for developmental programs. PMID: 25349408
  17. that p52 binds to the promoter of the GM-CSF-encoding gene (Csf2) and cooperates with c-Rel in the transactivation of this target gene. PMID: 24899500
  18. NF-kappaB p65/p52 signaling mediated the effects of GDNF on Bcl-2 and Bcl-w expressions PMID: 23590664
  19. Heterozygous mutations in NFKB2 cause a unique form of early-onset CVID that also presents with central adrenal insufficiency. PMID: 24140114
  20. p100 deficiency alone was insufficient for full induction of genes regulated by the alternative NF-kappaB pathway. PMID: 22880094
  21. RelB/NF-kappaB2 signaling positively and intrinsically regulates hematopoietic stem/progenitor cell self-renewal. PMID: 22290873
  22. the alternative the NF-kappaB pathway via the processing of p52 from p100 negatively regulates osteoblastic differentiation and bone formation by modifying bone morphogenetic protein activity. PMID: 22282470
  23. The p52/RelB noncanonical pathway is critical to sustain CXCL12/SDF1 production in order for cells to migrate toward chromatin architectural protein HMGB1. PMID: 22287708
  24. Nuclear factor-kappaB2/p100 promotes endometrial carcinoma cell survival under hypoxia in a HIF-1alpha independent manner. PMID: 21537326
  25. findings demonstrate the physiological relevance of the NF-kappaB2 p100 precursor protein in limiting the potentially detrimental effects of constitutive NF-kappaB2 signaling in lymphocytes PMID: 20363924
  26. Mice that were deficient in the alternative NF-kappaB pathway subunit p52 showed an impaired T-cell response against lymphocytic choriomeningitis virus infection. PMID: 20565293
  27. Results show that TNF limits RANKL- and TNF-induced osteoclast formation in vitro and in vivo by increasing NF-kappaB p100 protein accumulation in osteoclast precursors. PMID: 19770515
  28. Nfkb2 knockout mice develop chronic trichuriasis associated with elevated production of antigen-specific IFN-gamma but can recover IL-13 responses and resistance to infection if IL-12 responses are blocked. PMID: 12370384
  29. ligation of CD40 on transfected 293 cells is shown to trigger p52 production by stimulating p100 ubiquitylation and subsequent proteasome-mediated proteolysis PMID: 12374738
  30. mice injected with BAFF-neutralizing BR3-Fc protein showed reduced basal NF-kappaB2 activation PMID: 12387744
  31. NF-kappa B2 p100 has I kappa B-independent apoptotic activity and a unique tumour suppressor role PMID: 12389034
  32. role in Peyer's patch development along with RelB and p50, focusing on lymphotoxin and tumor necrosis factor signal transduction PMID: 12505990
  33. RelB has an effect on p100 processing, which is possibly regulated in a signal-dependent manner PMID: 12874295
  34. controls osteoclatogenesis PMID: 12939342
  35. NF-kappaB2 represses RelB in dendritic cells (DC) by a regulatory pathway that may function to prevent DC hyperactivation, with critical consequences for the regulation of adaptive immune responses. PMID: 14707043
  36. Results identify a novel type of NF-kappaB-binding site in IkappaB kinase alpha-dependent chemokine gene promoters that is preferentially recognized by RelB:p52 dimers. PMID: 15470505
  37. IkappaB kinase alpha has an essential role in the constitutive processing of NF-kappaB2 p100 PMID: 15677466
  38. RelB/p52 complexes have roles in mammary gland development and carcinogenesis PMID: 16260626
  39. CD14-mediated LPS signaling pathway may play a role in the regulation of NF-kappaB alternative splicing in the lungs after injury PMID: 16480837
  40. Akt plays a role in activation of basal and induced non-canonical NF-kappaB activity PMID: 16613850
  41. NF-kappaB2 (p100) limits nuclear translocation of NF-kappaB1-RelA and thereby functions as a regulatory 'brake' for the activation of naive T cells. PMID: 16732290
  42. NF-kappaB2 downstream of lymphotoxin beta receptor plays an important role in the regulation of central tolerance in an autoimmune regulator-dependent manner PMID: 17039258
  43. findings identify a physiological function of NF-kappaB2 in the development of medullary thymic epithelial cells and, thus, the control of self-tolerance induction PMID: 17046818
  44. Our genetic evidence confirms that p100 is required and sufficient as a fourth IkappaB protein for noncanonical NF-kappaB signaling downstream of NIK and IKK1 PMID: 17254973
  45. Increased p100/p52 disrupts normal mammary gland ductal development by a mechanism which may contribute to human breast cancer. PMID: 17261585
  46. activation of NF-kappaB2/p100 plays a crucial role in the Tax1-mediated transformation of T cells PMID: 17715223
  47. production of NF-kappaB2 p52 is not tumorigenic but predisposes mice to inflammatory autoimmune disease by repressing Bim expression PMID: 18281283
  48. inhibition of TRAF3 results in coordinated activation of both the p50-dependent and p52-dependent NF-kappaB activation pathways PMID: 18292232
  49. tightly controlled p100 processing and RelB activation is essential for normal B lymphopoiesis and lymphoid/myeloid lineage decision in bone marrow PMID: 18505785
  50. RIG-1 - MAVS interacts with cytoplasmic 100-kDa NF-kappa B2 complexes via a novel retinoic acid-inducible gene-I - NF- kappa B-inducing kinase signaling pathway PMID: 18550535

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Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Highly expressed in lymph nodes and thymus.
Database Links
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