Product Details

Purity

>85% (SDS-PAGE)

Target Names

Nr2c1

Uniprot No.

Q505F1

Alternative Names

Nr2c1; Tr2; Tr2-11; Nuclear receptor subfamily 2 group C member 1; Orphan nuclear receptor TR2; Testicular receptor 2; mTR2

Species

Mus musculus (Mouse)

Expression Region

1-590

Target Protein Sequence

MATIEEIAHQ IIDQQMGEIV TEQQTGQKIQ IVTALDHSTQ GKQFILANHE GSTPGKVFLT TPDAAGVNQL FFTSPDLSAP HLQLLTEKSP DQGPNKVFDL CVVCGDKASG RHYGAITCEG CKGFFKRSIR KNLVYSCRGS KDCVINKHHR NRCQYCRLQR CIAFGMKQDS VQCERKPIEV SREKSSNCAA STEKIYIRKD LRSPLAATPT FVTDSETARS AGLLDSGMFV NIHPSGIKTE PAMLMAPDKA ESCQGDLSTL ASVVTSLANL GKAKDLSHCG GDMPVVQSLR NGDTSFGAFH HDIQTNGDVS RAFDTLAKAL TPGESSACQS PEEGMEGSPH LIAGEPSFVE KEGPLLSESH VAFRLTMPSP MPEYLNVHYI GESASRLLFL SMHWALSIPS FQALGQENSI SLVKAYWNEL FTLGLAQCWQ VMNVATILAT FVNCLHSSLQ QDKMSPERRK SLMEHIFKLQ EFCNSMVKLC IDGHEYAYLK AIVLFSPDHP GLENMELIER FQEKAYVEFQ DYITRTYPDD TYRLSRLLLR LPALRLMNAT ITEELFFKGL IGNVRIDSVI PHILKMEPAD YNSQIIGHSL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.

Gene References into Functions

This study supports a role for Nr2c1 in defining the biphasic period of retinal development and specifically influencing the early phase of retinal cell fate. PMID: 28551284
Nr2c1 may be a candidate for mediating parallel adaptive changes in cranial neural sensory specializations such as the olfactory epithelium, retina and mystacial vibrissae and in non-neural craniofacial features including teeth PMID: 26712358
TR2/TR4 expression in sickle cell disease mice confers enhanced fetal hemoglobin synthesis and alleviated disease phenotypes PMID: 22042865
in adult erythroid cells, TR2/TR4 bind to the embryonic beta-type globin promoters but not to the adult beta-globin promoter. PMID: 21670149
TR2 may not play essential roles in spermatogenesis and normal testis development, function, and maintenance. Alternatively, the roles of TR2 may be redundant and could be played by other close members of the nuclear receptor superfamily (TR2 receptor) PMID: 12052874
DRED is a 540 kDa complex containing the nuclear orphan receptors TR2 and TR4, which form a heterodimer that binds to the epsilon and gamma globin promoter DR1 sites. TR2 & 4 mRNAs are expressed at all stages of murine and human erythropoiesis. PMID: 12093744
These results suggest a SUMOylation-dependent partitioning and differential coregulator recruitment contribute to the maintenance of a homeostatic supply of activating, Tr2, thus fine-tuning Oct4 expression and regulating stem-cell proliferation. PMID: 17187077
Silencing of the embryonic and fetal beta globin genes is delayed in erythroid cells of Tr2 and Tr4 null mutant mice, whereas in transgenic mice expressing dominant-negative TR4 (dnTR4), human embryonic epsilon-globin is activated in erythroid cells. PMID: 17431400
TR2 and TR4 orphan nuclear receptors repress Gata1 transcription. PMID: 17974920
Retinoic acid-stimulated sequential phosphorylation, PML recruitment, and SUMOylation of nuclear receptor TR2 to suppress Oct4 expression PMID: 18682553
Possible roles for TR2 and TR4 in early embryonic development by regulating key genes involved in stem cell self-renewal, commitment, and differentiation. PMID: 19131575
This is the first report identifying a deacetylase-independent function for HDAC3, which serves as a specific carrier molecule that targets a specifically phosphorylated protein to promyelocytic leukemia (PML) nuclear bodies. PMID: 19204783

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Subcellular Location

Nucleus. Nucleus, PML body. Note=Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-210 phosphorylation, to PML bodies for subsequent sumoylation.

Protein Families

Nuclear hormone receptor family, NR2 subfamily

Tissue Specificity

Isoform 1 is highly expressed in the adlumenal compartment of the seminiferous tubule of adult testes (at protein level) and in the eyes of newborn animals. Weakly expressed in other adult organs including the seminal vesicle, prostate, ovary, adrenal gla

Database Links

KEGG: mmu:22025

STRING: 10090.ENSMUSP00000089858

UniGene: Mm.107483

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Recombinant Mouse Nuclear receptor subfamily 2 group C member 1 (Nr2c1)

Product Details

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Target Background

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