Recombinant Mouse POU domain, class 3, transcription factor 4 (Pou3f4)

Code CSB-YP018399MO
MSDS
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Source Yeast
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Code CSB-EP018399MO
MSDS
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Source E.coli
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Code CSB-EP018399MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018399MO
MSDS
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Source Baculovirus
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Code CSB-MP018399MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pou3f4
Uniprot No.
Alternative Names
Pou3f4; Brn-4; Brn4; Otf9; POU domain; class 3; transcription factor 4; Brain-specific homeobox/POU domain protein 4; Brain-4; Brn-4; Octamer-binding protein 9; Oct-9; Octamer-binding transcription factor 9; OTF-9
Species
Mus musculus (Mouse)
Expression Region
1-361
Target Protein Sequence
MATAASNPYS ILSSSSLVHA DSAGMQQGSP FRNPQKLLQS DYLQGVPSNG HPLGHHWVTS LSDGGPWSST LATSPLDQQD VKPGREDLQL GAIIHHRSPH VAHHSPHTNH PNAWGASPAP NSSITSSGQP LNVYSQPGFT VSGMLEHGGL TPPPAAASTQ SLHPVLREPP DHGELGSHHC QDHSDEETPT SDELEQFAKQ FKQRRIKLGF TQADVGLALG TLYGNVFSQT TICRFEALQL SFKNMCKLKP LLNKWLEEAD SSTGSPTSID KIAAQGRKRK KRTSIEVSVK GVLETHFLKC PKPAAQEISS LADSLQLEKE VVRVWFCNRR QKEKRMTPPG DQQPHEVYSH TVKTDASCHD L
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain.
Gene References into Functions
  1. Brn4 substantially contributes to cochlear gap junction properties to maintain the proper EP in cochleae, similar to connexin-related deafness. PMID: 25259580
  2. Efnb2 expression was attenuated in Pou3f4 hemizygous null mutants relative to control. PMID: 25299585
  3. Results show three novel mutations in the POU3F4 gene resulting in profound hearing loss in both humans and mice. PMID: 23606368
  4. This study indicated a model whereby Pou3f4 in the otic mesenchyme establishes an Eph/ephrin-mediated fasciculation signal that promotes inner radial bundle formation. PMID: 22243746
  5. pou3f4 expression in inner ear might be under the control of distinct regulatory elements that fine-tune the spatio-temporal activity of this gene PMID: 21209840
  6. The effects of Brn-4 on the neuronal differentiation and development of neural stem cells, were investigated. PMID: 20524937
  7. a transcription factor targeted to the early mouse pancreas induces ectopic glucagon gene expression PMID: 11834725
  8. late-onset effect of Brn-4 deficiency on the hearing organ of the mouse. About one third of heterozygous female mice revealed late-onset profound deafness at the age of 1 year. PMID: 12062767
  9. lack of role in regulating Pax6 and Nkx2.2 PMID: 15031110
  10. Tbx1 and Brn4 function in signaling from the periotic mesenchyme to the otic vesicle to direct proper coiling of the cochlear duct. PMID: 18231833
  11. Brn4 regulate retinoic acid metabolic genes during cochlear morphogenesis PMID: 19476657

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Subcellular Location
Nucleus.
Protein Families
POU transcription factor family, Class-3 subfamily
Tissue Specificity
Brain specific.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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