Recombinant Mouse Palladin (Palld), partial

Code CSB-YP884164MO
MSDS
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Source Yeast
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Code CSB-EP884164MO
MSDS
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Source E.coli
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Code CSB-EP884164MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884164MO
MSDS
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Source Baculovirus
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Code CSB-MP884164MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Palld
Uniprot No.
Alternative Names
Palld; Kiaa0992; Palladin
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific May be required for the initiation of neural tube closure.
Gene References into Functions
  1. Study shows palladin is expressed with proper spatio-temporal pattern in the neural folds. It plays a crucial role in regulating mouse cranial neural tube closure by modulating cytoskeleton, proliferation, differentiation, apoptosis, and adhesion of neural epithelium PMID: 28340616
  2. these results show a new function for PALLD as a crucial regulator of the early phase of phagocytosis by elaborating dynamic actin polymerization and depolymerization PMID: 28739877
  3. Interactions with phosphatidylinositol 4,5-bisphosphate decrease the actin polymerizing activity of Ig domain 3 of palladin (Palld-Ig3). PMID: 27487483
  4. The aggregation of palladin(+/-) platelets was increased in response to low levels of thrombin, U46619, and collagen. This study also reports enhanced spreading of palladin(+/-) platelets on immobilized fibrinogen (Fg) and increased rate of clot retraction in platelet-rich plasma (PRP) containing palladin(+/-) platelets. PMID: 27865965
  5. The inhibition of palladin in myoblasts stimulates them to exit the cell cycle and express myogenic markers at the early phases of myogenesis, but retards the formation of multinucleated myotubes via the enhanced activation of myostatin. PMID: 25875253
  6. Suggesting that FSH signaling during Sertoli cell maturation regulates subcellular localization of palladin. PMID: 24989903
  7. Palladin is required for migration behavior and differentiation potential of cultured myoblast cells. PMID: 25194811
  8. High levels of palladin expression in cancer associated fibroblasts enhance their ability to remodel the extracellular matrix by regulating the activity of Cdc42. PMID: 23524582
  9. 140 kDa isoform of PALLD predominates in Sertoli cells, and that it is apparently cleaved, with the C-terminus localizing to the nucleus while the N-terminus remains cytoplasmic. PMID: 23559268
  10. Palladin mutations that disrupt actin binding show altered cellular distributions and morphology of actin in cells, revealing a functional requirement for the interaction between palladin and actin in vivo. PMID: 23806659
  11. upregulation of 85-90 kDa palladin isoform may play a role in the establishment of the tumor-associated fibroblasts phenotype, and thus in the formation of a desmoplastic tumor microenvironment PMID: 20436683
  12. Actin associated protein palladin is important for the early smooth muscle cell differentiation. PMID: 20877641
  13. palladin is dispensable for normal neurite outgrowth in mice PMID: 19730728
  14. The actin-associated protein palladin may play an important role in recruiting VASP to sites of actin filament growth, anchorage, and crosslinking. PMID: 14983521
  15. We propose that palladin is required for the initiation of neural tube closure and provides an important new candidate that may be implicated in the etiology of human NTDs. PMID: 15950489
  16. Here, we demonstrate that palladin is a binding partner for profilin, interacting with profilin via a poly proline-containing sequence in the amino-terminal half of palladin. PMID: 16367745
  17. palladin is crucial for definitive erythropoiesis and erythroblastic island formation PMID: 17431131
  18. expression analysis of the murine palladin isoforms PMID: 18924229
  19. Palladin is essential for expression of the full complement of contractile proteins necessary for optimal force development of smooth muscle cells derived from embryoid bodies PMID: 19015263
  20. results indicate that alpha-actinin, CLP36 and palladin form a protein complex and contribute to regulation of the actin cytoskeleton PMID: 19366376

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Subcellular Location
Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, myofibril, sarcomere, Z line. Cell projection, ruffle. Cell projection, podosome. Cell projection, lamellipodium. Cell projection, axon. Cell projection, growth cone.
Protein Families
Myotilin/palladin family
Tissue Specificity
Detected in both muscle and non-muscle tissues and cells (at protein level). Isoform 3 is widely expressed, isoform 4 is particularly abundant in tissues rich in smooth muscle and in the cardiac muscle and isoform 1 is detected in heart.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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