Recombinant Mouse Phosphoglycerate kinase 2 (Pgk2)

Code CSB-YP017859MO
MSDS
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Source Yeast
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Code CSB-EP017859MO
MSDS
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Source E.coli
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Code CSB-EP017859MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017859MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017859MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pgk2
Uniprot No.
Alternative Names
Pgk2; Pgk-2; Phosphoglycerate kinase 2; EC 2.7.2.3; Phosphoglycerate kinase; testis specific
Species
Mus musculus (Mouse)
Expression Region
1-417
Target Protein Sequence
MALSAKLTLD KVDLKGKRVI MRVDFNVPMK NNQITNNQRI KAAIPSIKHC LDNGAKSVVL MSHLGRPDGI PMPDKYSLEP VADELKSLLN KDVIFLKDCV GPEVEQACAN PDNGSIILLE NLRFHVEEEG KGKDSSGKKI SADPAKVEAF QASLSKLGDV YVNDAFGTAH RAHSSTVGVN LPQKASGFLM KKELDYFSKA LEKPERPFLA ILGGAKVKDK IQLIKNMLDK VNFMIIGGGM AYTFLKELKN MQIGASLFDE EGATIVKEIM EKAEKNGVKI VFPVDFVTGD KFDENAKVGQ ATIESGIPSG WMGLDCGPES IKINAQIVAQ AKLIVWNGPI GVFEWDAFAK GTKALMDEVV KATSNGCVTI IGGGDTATCC AKWGTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential for sperm motility and male fertility but is not required for the completion of spermatogenesis.
Gene References into Functions
  1. PGK2 is expressed differently in the testes of mouse and pigs according to developmental stage. PMID: 29407897
  2. These results identify multiple signaling and metabolic defects that are likely contributors to male infertility and the absence of progressive sperm motility seen in mice lacking GAPDHS. PMID: 29025010
  3. Data from studies in transgenic mice suggest that Pgk2 core promoter elements are required to direct demethylation of DNA and reconfiguration of nucleosomes in testis during sexual maturation and spermatogenesis. PMID: 24000349
  4. not required for the completion of spermatogenesis, but essential for sperm motility and male fertility PMID: 19759366
  5. PTBP2 is a trans-acting factor that helps to stabilize Pgk2 mRNA in male mouse germ cells. PMID: 17329592
  6. Data suggest that binding of PBX4, plus its coactivator PREP1, is a rate-limiting step leading to the initiation of tissue-specific transcription of the Pgk2 gene. PMID: 17875925
  7. Pgk2 crystal structures have been solved; the apoenzyme is in the "open" form; that is, the N-terminal domain that can bind 3-phosphoglycerate and the C-terminal domain that binds ATP are too far apart for the substrates to interact. PMID: 18004764
  8. t-KH-type splicing regulatory protein binds directly to a 93-nt sequence (designated the F1 region) of the 3'-UTR of the Pgk2 mRNA and destabilizes Pgk2 mRNA constructs in testis extracts. PMID: 19015122

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Subcellular Location
Cytoplasm.
Protein Families
Phosphoglycerate kinase family
Tissue Specificity
Testis and sperm. Localized on the principle piece in the sperm (at protein level). Testis-specific.
Database Links

KEGG: mmu:18663

STRING: 10090.ENSMUSP00000033585

UniGene: Mm.717

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