Recombinant Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (Plod1)

Code CSB-YP882597MO
MSDS
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Source Yeast
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Code CSB-EP882597MO
MSDS
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Source E.coli
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Code CSB-EP882597MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882597MO
MSDS
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Source Baculovirus
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Code CSB-MP882597MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Plod1
Uniprot No.
Alternative Names
Plod1; Plod; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1; EC 1.14.11.4; Lysyl hydroxylase 1; LH1
Species
Mus musculus (Mouse)
Expression Region
19-728
Target Protein Sequence
KD DAKLEDNLLV LTVATKETEG FRRFKRSAQF FNYKIQSLGL GEDWSVDGGP AAAGGGQKVR LLKKALEKHA DKEDLVILFV DSYDVVFASG PRELLKKFQQ AKSQVVFSAE EHIYPDRRLE AKYPTVPDGK RFLGSGGFIG YAPSLSKLVA EWEGQDSDSD QLFYTKIFLN PEKREQINIS LDHRCRIFQN LDGALDEVVL KFEMGHVRAR NLAYDTLPVV VHGNGPTKLQ LNYLGNYIPR FWTFETGCTV CDEGLRSLKG IGDEALPTVL VGVFIEQPTP FLSLFFLRLL RLRYPQKQMR LFIHNQERHH KLQVEQFLAE HGSEYQSVKL VGPEVRMANA DARNMGADLC RQDQTCTYYF SVDADVALTE PNSLRLLIEQ NKNVIAPLMT RHGRLWSNFW GGLSADGYYA RSEDYVDIVQ GRRVGVWNVP YISNIYLIKG SALRAELQNV DLFHYSKLDS DMSFCANVRQ QEVFMFLTNR HTFGHLLSLD NYQTTHLHND LWEVFSNPED WKEKYIHENY TKALAGKLVE TPCPDVYWFP IFTEAACDEL VEEMEHYGQW SLGDNKDNRI QGGYENVPTI DIHMNQITFE REWHKFLVEY IAPMTEKLYP GYYTRAQFDL AFVVRYKPDE QPSLMPHHDA STFTVNIALN RVGEDYEGGG CRFLRYNCSV RAPRKGWALL HPGRLTHYHE GLPTTKGTRY IAVSFVDP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Gene References into Functions
  1. Plod1(-/-) mice offer an in vivo model for systematic analysis of the tissue-specific consequences of the lack of LH1 activity PMID: 17197443
Subcellular Location
Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.
Tissue Specificity
Highly expressed in the liver, heart, lung, skeletal muscle and kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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