Recombinant Mouse Protein NLRC5 (Nlrc5), partial

Code CSB-YP015863MO
MSDS
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Source Yeast
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Code CSB-EP015863MO
MSDS
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Source E.coli
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Code CSB-EP015863MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015863MO
MSDS
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Source Baculovirus
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Code CSB-MP015863MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nlrc5
Uniprot No.
Alternative Names
Nlrc5; Protein NLRC5
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms.
Gene References into Functions
  1. Taken together, NLRC5 promotes inflammation and fibrosis during diabetic nephropathy progression partly through the effects on NF-kappaB and TGF-beta/Smad pathways. PMID: 29070585
  2. NLRC5 knockout mice fed with high fat showed accelerated fibrosis and inflammation response by promoting alpha-SMA, Collagen I, Collagen III, TLR4/MyD88, phosphorylated IKKalpha, IkappaBalpha and NF-kappaB expression. PMID: 28499247
  3. NLRC5 knockout in mice exhibited greater hepatic injury after hepatic ischemia/reperfusion (I/R) injury. PMID: 28784305
  4. The observed increases in viral titers in NLRC5-deficient mice correlated with impaired effector CD8(+) T cell responses. Although NLRC5-deficient mice were defective at clearing the virus, they did not show an increase in morbidity or mortality following influenza virus infection because of other compensatory immune mechanisms. PMID: 28615208
  5. NLRC5 regulates TGFB1-induced proliferation and activation of hepatic stellate cells during hepatic fibrosis. PMID: 26592197
  6. NLRC5 is differentially expressed in hepatic tissues and hepatic stellate cells during hepatic fibrosis and its reversal PMID: 26806094
  7. NLRC5 expression in T cells is required to protect them from NK-cell-mediated elimination upon inflammation. PMID: 26861112
  8. NLRC5 and CIITA thus emerge as paradigms for a novel class of transcriptional regulators dedicated for transactivating extremely few, phylogenetically related genes. PMID: 25811463
  9. Unusual structural features revealed by the solution NMR structure of the NLRC5 caspase recruitment domain. PMID: 24815518
  10. Data conclude that Nlrc5 is important in the regulation of MHC-I expression by reducing H3K27me3 on MHC-I promoter and joins CIITA as an NLR subfamily that controls MHC gene transcription. PMID: 22645137
  11. NLRC5 is required for both constitutive and inducible histocompatibility class I gene expression and host defense. PMID: 22711889
  12. NLRC5 ablation reduces MHC class I expression, and enhances IKK and IRF3 phosphorylation in response to TLR stimulation or viral infection. PMID: 22473004
  13. NLRC5-deficient mice showed a profound defect in the expression of MHC class I genes and a concomitant failure to activate L. monocytogenes-specific CD8(+) T cell responses, including activation, proliferation and cytotoxicity. PMID: 22491475
  14. NLRC5 overexpression promotes interleukin (IL)-1beta production via caspase-1 and is dispensable for cytokine induction in virus and bacterial infections under physiologic conditions. PMID: 21148033
  15. NLRC5 expression is critical for lipopolysaccharide-induced interleukin (IL)-10 production in RAW264.7 macrophages. PMID: 20610642
  16. Study identifies NLRC5 as a negative regulator that blocks two central components of the NF-kappaB and type I interferon signaling pathways and suggest an important role for NLRC5 in homeostatic control of innate immunity. PMID: 20434986

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Subcellular Location
Cytoplasm.
Protein Families
NLRP family
Tissue Specificity
Expressed in spleen, thymus and lung.
Database Links

KEGG: mmu:434341

UniGene: Mm.334720

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