Recombinant Mouse Regulator of G-protein signaling 7 (Rgs7)

Code CSB-YP019659MO
MSDS
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Source Yeast
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Code CSB-EP019659MO
MSDS
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Source E.coli
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Code CSB-EP019659MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019659MO
MSDS
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Source Baculovirus
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Code CSB-MP019659MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rgs7
Uniprot No.
Alternative Names
Rgs7; Regulator of G-protein signaling 7; RGS7
Species
Mus musculus (Mouse)
Expression Region
1-469
Target Protein Sequence
MAQGNNYGQT SNGVADESPN MLVYRKMEDV IARMQDEKNG IPIRTVKSFL SKIPSVFSGS DIVQWLIKNL TIEDPVEALH LGTLMAAHGY FFPISDHVLT LKDDGTFYRF QTPYFWPSNC WEPENTDYAV YLCKRTMQNK ARLELADYEA ESLARLQRAF ARKWEFIFMQ AEAQAKVDKK RDKIERKILD SQERAFWDVH RPVPGCVNTT EVDIKKSSRM RNPHKTRKSV YGLQNDIRSH SPTHTPTPET KPPTEDELHQ QIKYWQIQLD RHRLKMSKVA DSLLSYTEQY VEYDPFLVPP DPSNPWLSDD TTFWELEASK EPSQQRVKRW GFGMDEALKD PVGREQFLKF LESEFSSENL RFWLAVEDLK RRPIREVPSR VQEIWQEFLA PGAPSAINLD SKSYDKTTQN VKEPGRYTFE DAQEHIYKLM KSDSYPRFIR SSAYQELLQA KRKGKTLTSK RLTSLVQSY
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS7/GNB5 dimer enhances GNAO1 GTPase activity. May play a role in synaptic vesicle exocytosis. Modulates the activity of potassium channels that are activated by GNAO1 in response to muscarinic acetylcholine receptor M2/CHRM2 signaling.
Gene References into Functions
  1. Stimulation of the ERK pathway in Gnb5(-/-) cells by epidermal growth factor restored M3R-stimulated insulin release to near normal levels. Identification of the novel role of Gbeta5-R7 in insulin secretion may lead to a new therapeutic approach for improving pancreatic beta-cell function PMID: 28687610
  2. This study identifies RGS7 as a novel regulator of Mu Opioid Receptor signaling by dissecting its circuit specific actions and pinpointing its role in regulating morphine reward by controlling the activity of nucleus accumbens neurons. PMID: 26364547
  3. R7-binding protein had a strong inhibitory effect on homo-oligomerization of RGS7. PMID: 26895961
  4. Association with the plasma membrane is sufficient for potentiating catalytic activity of RGS7 and RGS9-2 proteins. PMID: 26811338
  5. results demonstrate a novel role of RGS7 in hippocampal synaptic plasticity and memory formation PMID: 24755289
  6. GPR158 is an essential regulator of RGS7 in the native nervous system with a critical role in controlling its expression, membrane localization, and catalytic activity PMID: 25792749
  7. Expression of the Gbeta5/R7-RGS protein complex in pituitary and pancreatic islet cells. PMID: 22322946
  8. This study demonistrated that the subcellular localization of RGS7 in neurons and found that a significant fraction is present in cytoplasmic puncta. PMID: 22640015
  9. RGS7 and RGS11 are the key GTPase accelerating proteins in the mGluR6 pathway of retinal rod ON bipolar cells that set the sensitivity and time course of light-evoked responses. PMID: 22547806
  10. Defective retinal depolarizing bipolar cells in regulators of G protein signaling (RGS) 7 and 11 double null mice. PMID: 22371490
  11. R7BP contributes significantly to the nuclear localization of endogenous G beta(5)/regulator of G protein signaling 7 complex in brain PMID: 20100282
  12. study found that RGS7-Gbeta5 complexes bound to G protein-gated potassium channels and facilitated their functional coupling to GABA(B) receptors in neurons PMID: 20453851
  13. Delivery of RGS7/Gbeta5 to dendrites of ON-bipolar cells occurs independently of its association with R7BP. These findings provide a new mechanism for adapter-independent targeting of RGS/Gbeta5 complexes. PMID: 18842904
  14. RGS7 staining is present in dendritic tips of all rod ON-retinal bipolar cell. Genetic disruption of RGS7 produced delays in the ON-BPC-derived electroretinogram b-wave, but no changes in the photoreceptor-derived a-wave. PMID: 19535587

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Subcellular Location
Cytoplasm, cytosol. Cytoplasm. Cell membrane. Membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity
Detected in brain (at protein level).
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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