Recombinant Mouse Rho GTPase-activating protein 22 (Arhgap22)

Code CSB-YP805918MO
MSDS
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Source Yeast
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Code CSB-EP805918MO
MSDS
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Source E.coli
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Code CSB-EP805918MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805918MO
MSDS
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Source Baculovirus
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Code CSB-MP805918MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arhgap22
Uniprot No.
Alternative Names
Arhgap22; Rho GTPase-activating protein 22; Rho-type GTPase-activating protein 22; p68RacGAP
Species
Mus musculus (Mouse)
Expression Region
1-702
Target Protein Sequence
MLPTASSKRR TFAARYFTRS KSLVMGEQSR SPGRPLVPHK LGPVLKAGWL RKQRSIMKNW QQRWFVLRGD QLFYYKDKDE SKPQGFISLQ GTQVTELLPD PEDPGKHLFE ITPGGATERE KVPANPEALL LMASSQRDME DWVQAIRRVI WAPLGRGIFG QRLEDTVHHE RKFGPRLAPL LVEQCVDFIR ERGLSEEGLF RMPGQANLVR DLQDSFDCGE KPLFDSTTDV HTVASLLKLY LRELPEPVIP FARYEDFLSC AQLLTKDEGE GTVELAKQVS NLPQANYNLL RYICKFLDEV QAHSDVNKMS VQNLATVFGP NILRPQIEDP VTIMEGTSLV QHLMTVLIRK HGQLFAATSL EEPASPHGTV EWGSEEVTRD HRGEPGSPGL PTHRTSSLDG PAAAVLSRTS PPRLGSQTGP AATSPGKKMH TLPVWKSSFR QQGSRSESPK GVNSSLEVPI ISSGGNWLIN GLSSLRSHRR ASSGDRLKDT GSAQRLSTYD NVPPSSQFSS TASVASTSWS VASSSREASV SSCTACRASN SSACSSLHTE WALEPSPLPS SSEGHQSPDL GHSLDEPCVG SGSSEPNDPG SPTQAHVRRC RALQGQVAEL RAELCQQRTE YKRSLKSIEE GSADLRKQMS RLEEELDQER KKYAMLEIKL RNSERAREDA ERRNQLLQRE MEEFFSTLGS LTTGTKGSRA PE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.
Gene References into Functions
  1. a multifunctional regulatory protein with Rac1-specific GTPase-activating activity; regulates transcriptional activity of the endothelin-1 promoter; involved in the signal transduction pathway regulating endothelial cell capillary tube formation PMID: 14966113
Subcellular Location
Cytoplasm. Nucleus. Note=Mainly cytoplasmic. Some fraction is nuclear.
Tissue Specificity
Predominantly present in endothelial cells (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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