Recombinant Mouse Secretogranin-3 (Scg3)

Code CSB-YP020809MO
MSDS
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Source Yeast
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Code CSB-EP020809MO
MSDS
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Source E.coli
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Code CSB-EP020809MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020809MO
MSDS
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Source Baculovirus
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Code CSB-MP020809MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Scg3
Uniprot No.
Alternative Names
Scg3; Secretogranin-3; Secretogranin III; SgIII
Species
Mus musculus (Mouse)
Expression Region
23-471
Target Protein Sequence
FPKPEGSQ DKSLHNRELS AERPLNEQIA EAEADKIKKA FPSESKPSES NYSSVDNLNL LRAITEKETV EKERQSIRSP PFDNQLNVED ADSTKNRKLI DEYDSTKSGL DHKFQDDPDG LHQLDGTPLT AEDIVHKIAT RIYEENDRGV FDKIVSKLLN LGLITESQAH TLEDEVAEAL QKLISKEANN YEETLDKPTS RTENQDGKIP EKVTPVAAVQ DGFTNRENDE TVSNTLTLSN GLERRTNPHR EDDFEELQYF PNFYALLTSI DSEKEAKEKE TLITIMKTLI DFVKMMVKYG TISPEEGVSY LENLDETIAL QTKNKLEKNT TDSKSKLFPA PPEKSQEETD STKEEAAKME KEYGSLKDST KDDNSNLGGK TDEATGKTEA YLEAIRKNIE WLKKHNKKGN KEDYDLSKMR DFINQQADAY VEKGILDKEE ANAIKRIYSS L
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Member of the granin protein family that regulates the biogenesis of secretory granules. Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA. May also play a role in angiogenesis. Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway.
Gene References into Functions
  1. This review summarizes our current knowledge of Scg3 as a regulatory protein of secretory granules, highlights its new role as a highly disease-selective angiogenic factor, and envisions Scg3 inhibitors as "selective angiogenesis blockers" for targeted therapy of diabetic retinopathy.[ review] PMID: 28856381
  2. we show by RNA silencing that CPE and SgIII play a synergistic role in the trafficking of POMC to granules of the regulated secretory pathway in AtT20 cells PMID: 26646096
  3. SgIII knockdown impairs intracellular retention of chromogranin A and proopiomelanocortin in AtT-20 cells. PMID: 23171199
  4. The robust expression of SgIII in astrocytes and its regulation in the injured brain suggest both intracellular and extracellular roles for this glial granin in the physiology and repair/damage of neuronal circuits. PMID: 19892786
  5. SgIII directly binds to cholesterol components of the secretory granule membrane and targets chromogranin A to secretory granules in pituitary and pancreatic endocrine cells PMID: 14597614
  6. SgIII and CPE form the separate functional sorting complex by anchoring to cholesterol-rich SG membranes, and POMC-derived peptides are transferred from CPE to SgIII, and subsequently to CgA. PMID: 16219686

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Subcellular Location
Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted.
Tissue Specificity
Expressed in various brain areas, with highest levels in the arcuate nucleus and the lateral hypothalamic area, as well as the paraventricular nucleus and the ventromedial hypothalamus (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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