Product Details

Purity

>85% (SDS-PAGE)

Target Names

Srrm4

Uniprot No.

Q8BKA3

Alternative Names

Srrm4; Serine/arginine repetitive matrix protein 4; Neural-specific serine/arginine repetitive splicing factor of 100 kDa; Neural-specific SR-related protein of 100 kDa; nSR100

Species

Mus musculus (Mouse)

Expression Region

1-608

Target Protein Sequence

MASLQQGEKQ LFEKFWKGTF KAVATPRPES IIVASITARK PMPRTEPQSS LLLPDQDGPS EKLGQHLAPE ALGTNSWGRE KACRELDPAR AHSASQDRDP TPPPSSRGKK KKKKSTRKKR RRSPSYSPSP VKKKKKKSSK KHKRHRSFSK KRRHSSCSPK SKRREEKRHK KQSRSRKSHR HRHHRCPSRS QSSELRSPSC ESRHRGRSPE EGRKSRRTHS RRCSKNHCKV SPDARSSHLP SQPLPRLGFL SARGVITGSG SAADLFSKSA SPLAATRGRS QEYDSGNDTS SPPSTQTSSA RSRGQEKGSP GGDLSKSRDL NCGNTSDSGN SFTTSSPQNK GAVLETVSPA CRSRESRGFQ SPCLQCAEVK KSSLVPSTAR SSPIKECSRS SSYTSTRSSS PSSRSPNPRA SPRYTRSRST SSEKRSYSRS PSYSSKSGKR SPPSRSSRSR RSPSYSRYSP SRERDLKYGE KEPQPRERAR RRRRSYSPMR KRRRDSPSHL EARRITSARK RPIPYYRPSP SSSSSLSSAS SWYSSSSSSS SSSSRSPSRS YSRSRSPSRS HSSRSQTRSR TRTSRSSSSR SLSLGSRSRS RNRSLSYSSA ESYASTRR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Splicing factor specifically required for neural cell differentiation. Acts in conjunction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promotes neural-specific exon inclusion in many genes that function in neural cell differentiation. Required to promote the inclusion of neural-specific exon 10 in nPTB/PTBP2, leading to increased expression of neural-specific nPTB/PTBP2. Also promotes the inclusion of exon 16 in DAAM1 in neuron extracts. Promotes alternative splicing of REST transcripts to produce REST isoform 2 (REST4) with greatly reduced repressive activity, thereby activating expression of REST targets in neural cells. Plays an important role during embryonic development as well as in the proper functioning of the adult nervous system. Regulates alternative splicing events in genes with important neuronal functions.

Gene References into Functions

Results suggest that SRRM4 controls neurite outgrowth through regulation of alternative splicing of protrudin transcripts. PMID: 28106138
Neuronal activity-dependent microexons are nSR100 targets and are misregulated in autism. PMID: 27984743
Loss of nSR100 impairs development of the central and peripheral nervous systems in part by disrupting neurite outgrowth, cortical layering in the forebrain, and axon guidance in the corpus callosum PMID: 25838543
findings show that nSR100 plays a role in the alternative splicing of REST in small cell lung cancer (SCLC); study provides new insight into the role of nSR100 in the expression of sREST, possibly in the pathogenesis of SCLC PMID: 23928058
required for the continued survival and differentiation of inner and vestibular hair cells past a set point in their development PMID: 11950537
Srrm4 function is critical in the hearing and balance organs, but not in all neural tissues. Srrm4 is the first alternative-splicing regulator to be associated with hearing. Analysis of bv mice provides exon-level insights into hair-cell development. PMID: 23055939
role for nSR100 in the inhibition of REST activity, blocking nSR100 expression in the developing mouse brain impairs neurogenesis PMID: 21884984
Study identified the neural-specific SR-related protein of 100 kDa (nSR100)& shows that nSR100 regulates an extensive network of brain-specific alternative exons enriched in genes that function in neural cell differentiation. PMID: 19737518

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Subcellular Location

Nucleus.

Protein Families

NSR100 family

Tissue Specificity

Specifically expressed in neuronal cells (at protein level). Expressed in adult nervous system and sensory organ tissues.

Database Links

KEGG: mmu:68955

STRING: 10090.ENSMUSP00000075488

UniGene: Mm.146779

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Recombinant Mouse Serine/arginine repetitive matrix protein 4 (Srrm4)

Product Details

Customer Reviews and Q&A

Target Background

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