Recombinant Mouse Sperm equatorial segment protein 1 (Spesp1)

Code CSB-YP022533MO
MSDS
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Source Yeast
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Code CSB-EP022533MO
MSDS
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Source E.coli
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Code CSB-EP022533MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022533MO
MSDS
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Source Baculovirus
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Code CSB-MP022533MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Spesp1
Uniprot No.
Alternative Names
Spesp1; Sperm equatorial segment protein 1
Species
Mus musculus (Mouse)
Expression Region
19-399
Target Protein Sequence
YP TITVLPDEEQ NLNHYVHILQ NLIMSVPTKE QDLGKKLSSS RTVDSAEPRS LASKVLLTPG LVSAQDVTPE SDVLIRPVDE TTNSRTRGFT LRRRRTQSTA FWSIRPNNIS VVLRTEEPFI EKEPEPELES SRLPTEPEPE LEPEPEPVAE SRQMSEPEEE LVTSTTPNKE LTGTSRISSM ATQPANTQAT RITVTVKTTS TMDVSTDSED VPQLSGQSEI PSAEDLPGRH SLNTRHEDIL KKISNINAEI QQGLLGGNNS PEFKEFIKAS REHLKRSLAL AAAAEHKLEQ MYGSNVFPEG RTSDPDNDME MIINMLYNSR SKLSDYFNIK RVPSELREKA SVVNAELRKI LCVDQVEMQS LIKKLLSNNM KILNILNVP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in fertilization ability of sperm.
Gene References into Functions
  1. The present study identified SPESP1 in the acrosome as the primary binding target of Peanut agglutinin (PNA) in the mouse testis. Further defining the specific lectin-glycoprotein relationships in individual cells will enhance the value of lectin histochemistry. PMID: 27539077
  2. These findings are consistent with the hypothesis that SPSPESP1 undergoes significant glycosylation in the testis and that the majority of these glycoconjugates are removed by the time sperm reach the caput epididymis. PMID: 25761597
  3. SPESP1 is necessary to produce the fully 'fusion competent' sperm. PMID: 20375058
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, acrosome.
Protein Families
SPESP1 family
Tissue Specificity
Testis specific.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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