Recombinant Mouse Spermatogenesis-associated protein 16 (Spata16)

Code CSB-YP804919MO
MSDS
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Source Yeast
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Code CSB-EP804919MO
MSDS
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Source E.coli
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Code CSB-EP804919MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804919MO
MSDS
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Source Baculovirus
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Code CSB-MP804919MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Spata16
Uniprot No.
Alternative Names
Spata16; Spermatogenesis-associated protein 16
Species
Mus musculus (Mouse)
Expression Region
1-571
Target Protein Sequence
MDSGKSRSLE TTVKRVYRDL LLPKINTSKK MSTLTNSPSS LEGTPGIKKN SGEMQAEASA ERVKLTKSIK EKQNNELEKV ALKRKADSEE KLVGKKEAKI MELDNQLVTT VPLPHIPLKN IMDVEMKLVY VDEEDVSYEF AQPNMCLGLQ ATGQTATTMP PASPRDLTTL PQIDKWLQVA LKDASSCYRQ KKYAVAAGQF RTALELCSKG AALGKPFEAH AEDIASIASF IETKLVTCYL RMRKPDLALN HAHRSIVLNP AYFRNHLRQA AVFRCLERYS EAARSAMIAD YMFWLCGGSE HSVSKLIKLY WQAMIEEAIT RAEAFSVMYT PFATRIKPEN IEKVKEVFMR THPTYVDCIY TDTQGLHVLP QTADWSCFPP QQYLLTLGFK NKEDGKFLEK VSSRKLPTYT EHKTPFSPLT REDTVRHMET VGKRILPILD FIRSTQLNGN FHACSGVMEK LHYASLLSRL QRVKEQAQVI NQAMAELATV PYLQDISQQE AELLQSLMAD AMDTLEGKKS DKERVWNTIQ KVGRIEDFLY QLEDSFLKTK KLRTARRQKT KMKRLQTVQQ N
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion.
Gene References into Functions
  1. The point mutation in Spata16 was not essential for male fertility; however, deletion of the fourth exon of Spata16 resulted in infertile male mice due to spermiogenic arrest but not globozoospermia. This study demonstrates that Spata16 is indispensable for male fertility in mice, as well as in humans. PMID: 29065458
Subcellular Location
Golgi apparatus. Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Shift from Golgi to sperm acrosome.
Protein Families
SPATA16 family
Tissue Specificity
Expressed in spermatocytes and round and elongated spermatids in the seminiferous tubules.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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