Recombinant Mouse Sulfiredoxin-1 (Srxn1)

Code CSB-YP872019MO
MSDS
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Source Yeast
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Code CSB-EP872019MO
MSDS
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Source E.coli
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Code CSB-EP872019MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872019MO
MSDS
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Source Baculovirus
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Code CSB-MP872019MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Srxn1
Uniprot No.
Alternative Names
Srxn1; Npn3; Srx; Sulfiredoxin-1; EC 1.8.98.2; Neoplastic progression protein 3
Species
Mus musculus (Mouse)
Expression Region
1-136
Target Protein Sequence
MGLRAGGALR RAGAGPGAPE GQGPGGAQGG SIHSGCIATV HNVPIAVLIR PLPSVLDPAK VQSLVDTILA DPDSVPPIDV LWIKGAQGGD YYYSFGGCHR YAAYQQLQRE TIPAKLVRST LSDLRMYLGA STPDLQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase.
Gene References into Functions
  1. sulfiredoxin is degraded by Lon in a manner dependent on PrxIII hyperoxidation state. PMID: 26236015
  2. Srx is one of the critical components that contribute to mouse skin tumorigenesis in vivo. PMID: 24503444
  3. data provide in vivo evidence that loss of Srx renders mice resistant to azoxymethane/dextran sulfate sodium-induced colon carcinogenesis, suggesting that Srx has a critical oncogenic role in cancer development PMID: 23393226
  4. The concerted action of PrxIII and Srx is important for protection against pyrazole-induced oxidative stress arising from the convergent induction of CYP2E1-derived and ER stress-derived ROS in mitochondria. PMID: 22490042
  5. Srx functions as a novel component to maintain the balance between H2O2 production and elimination PMID: 22086924
  6. NO-mediated Srx up-regulation is mediated by the transcription factor nuclear factor erythroid 2-related factor (Nrf2) and the NO/Srx pathway inhibits generation of reactive oxygen species. PMID: 21466852
  7. Sulfiredoxin protects mice from lipopolysaccharide-induced endotoxic shock PMID: 21083423
  8. LPS-mediated Srx induction is dependent on both AP-1 and Nrf2, which is regulated by Nox2-derived reactive oxygen species. PMID: 20826812
  9. Synaptic activity upregulated the thioredoxin-peroxiredoxin reactivating gene sulfiredoxin. PMID: 18344994

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Subcellular Location
Cytoplasm.
Protein Families
Sulfiredoxin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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