Recombinant Mouse TCDD-inducible poly [ADP-ribose] polymerase (Tiparp)

Code CSB-YP806010MO
MSDS
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Source Yeast
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Code CSB-EP806010MO
MSDS
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Source E.coli
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Code CSB-EP806010MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806010MO
MSDS
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Source Baculovirus
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Code CSB-MP806010MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Tiparp
Uniprot No.
Alternative Names
TiparpProtein mono-ADP-ribosyltransferase TIPARP; EC 2.4.2.-; ADP-ribosyltransferase diphtheria toxin-like 14; ARTD14; TCDD-inducible poly [ADP-ribose] polymerase
Species
Mus musculus (Mouse)
Expression Region
1-657
Target Protein Sequence
MEVETTEPEP DCVVQPPSPS DDFSCQMRIS EKISPLKTCF KKKQEQKRLG TGTLRSLRPI LNTLLESGSL DGVFRARDQN RDESSLHEHI VKKPLEINPS CPPAENSMPV LIPDGTNVEG QLPEAHPSTD APEQGVPIQD HSFPPETISG TVADSTTGHF QTDLLHPVSG DVPTSPDCVD KVMDYVPGAF QDNSFTIQYI LDTSDKLSTE LFQDKSEEAS LELVFELVNQ LQYHTHQENG IEICMDFLQG TCIYGRDCLK HHTVLPYHWQ IKRTTTQKWQ SVSNDSQEHL ERFYCNPEND RMRMKYGGQD FWADLNAMTV FETTEFDQLR RLSTPPCSNS NSIYHTFWKF FCRDHFGWRE YPESVVRLIE EANSRGLKEV RFMMWNNHYI LHNSFFRREI KRRPLFRSCF ILIPYLQTLG GVPTQASLPL EATSSQIICP DGVTSANFYP ETWVYMHPSQ DFIQVPVSAE DKSYRIIYNL FHKTVPEFKY RILQILRVQN QFLWEKYKRK KEYMNRKMSG RDRIINERHL FHGTSQDVVD GICKHNFDPR VCGKHATMFG QGSYFAKKAS YSHNFSKKSS KGVHFMFLAK VLTGRYTMGS HGMRRPPPVN PGSVTSDLYD SCVDNFFEPQ IFVIFNDDQS YPYFVIQYEE VSNTVSI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate, aspartate and cysteine residues on target proteins. Acts as a negative regulator of AHR by mediating mono-ADP-ribosylation of AHR, leading to inhibit transcription activator activity of AHR (Probable).
Gene References into Functions
  1. this study shows that aryl hydrocarbon receptor upregulates expression of TIPARP, which in turn causes downregulation of the IFN type I response PMID: 27089381
  2. these data identify a new mechanism of LXR regulation that involves TIPARP, ADP-ribosylation and MACROD1. PMID: 26814197
  3. Loss of Tiparp increases sensitivity to dioxin toxicity and lethality. PMID: 25975270
  4. Knockdown of TiPARP, but not AHRR, increased 2,3,7,8-tetrachlorodibenzo-p-dioxin - induced CYP1A1 mRNA and AHR protein levels. PMID: 24806346
Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitously expressed.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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