Recombinant Mouse Visual system homeobox 2 (Vsx2)

Code CSB-YP025939MO
MSDS
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Source Yeast
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Code CSB-EP025939MO
MSDS
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Source E.coli
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Code CSB-EP025939MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025939MO
MSDS
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Source Baculovirus
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Code CSB-MP025939MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Vsx2
Uniprot No.
Alternative Names
Vsx2; Chx10; Visual system homeobox 2; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10
Species
Mus musculus (Mouse)
Expression Region
1-361
Target Protein Sequence
MTGKAGEALS KPKSETVAKS TSGGAPARCT GFGIQEILGL NKEPPSSHPR AALDGLAPGH LLAARSVLSP AGVGSMGLLG PGGLPGFYTQ PTFLEVLSDP QSVHLQPLGR ASGPLDTSQT ASSDSEDVSS SDRKMSKSAL NQTKKRKKRR HRTIFTSYQL EELEKAFNEA HYPDVYAREM LAMKTELPED RIQVWFQNRR AKWRKREKCW GRSSVMAEYG LYGAMVRHSI PLPESILKSA KDGIMDSCAP WLLGMHKKSL EAAAESGRKP EVERQALPKL DKMEQEERAP EAQAAISQEE LRENSIAALR AKAQEHSTKV LGTVSGPDSL ARNAEKPEEE DATEEDRPAE KLSPPQLEDM A
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a transcriptional regulator through binding to DNA at the consensus sequence 5'-[TC]TAATT[AG][AG]-3' upstream of gene promoters. Plays a significant role in the specification and morphogenesis of the sensory retina. Mediates differentiation of V2a interneurons by repression of motor neuron gene transcription, via competitively binding to response elements that are activated by the ISL1-LHX3 complex, such as VSX1. Acts as a positive transcriptional regulator of NXNL1; regulation is significantly increased in synergy with VSX1. Acts as a negative transcriptional regulator of MITF. Represses SAG transcription by competitive inhibition of ISL1-LHX3 response elements. Binds to the photoreceptor conserved element-1 (PCE-1) in the promoter of rod photoreceptor arrestin SAG and acts as a transcriptional repressor. Involved in the development of retinal ganglion cells (RGCs) which leads to release of SHH by RGCs, promoting Hedgehog signaling and subsequent proliferation of retinal progenitor cells. Participates in the development of the cells of the inner nuclear layer, by promoting postnatal differentiation of bipolar cells with a comparable inhibition of rod cell differentiation. May play a role in the maintenance of neural retina identity during development by regulation of canonical Wnt genes and CTNNB1 localization, suggesting a role in the regulation of canonical Wnt signaling.
Gene References into Functions
  1. Vsx2 represses Mitf in a cell-autonomous way. It also uses cell-nonautonomous mechanisms to regulate progenitor properties in the embryonic retina. Vsx2's role in regulating Mitf is in part separable from its role in promoting proliferation. PMID: 25927996
  2. The results of this study suggested that Lhx3/Chx10 medullary reticular formation neurons are involved in locomotion. PMID: 24027269
  3. Our data suggest that the phenotypic severity of the CVC mutant depends on the weakened DNA binding activity elicited by the CVC mutation and a previously unknown protein interaction between Vsx2 and its regulatory target Mitf. PMID: 23028343
  4. analysis of the regulatory role of a conserved motif adjacent to the homeodomain of Hox10 proteins PMID: 22721778
  5. Reducing GDF11 levels might promote restoration of retinal development in the microophthalmic Vsx2 mutant mouse. PMID: 22183392
  6. Data demonstrate that Hox10 genes play a critical role in the developing kidney. PMID: 21858105
  7. Blimp1 may play a role in photoreceptor development by repressing genes involved in bipolar cell fate specification and retinal cell proliferation in differentiating precursors; Blimp1 can bind to the Chx10 enhancer and repress Chx10 enhancer activity PMID: 20463215
  8. CHX10 regulates RdCVF promoter activity in the inner retina. PMID: 19843539
  9. results have shown that the Hox10 and Hox11 paralogous genes are global regulators of the lumbosacral region of the axial skeleton and are integral in patterning principal limb elements PMID: 12869760
  10. Delay of the normal temporal expression of genes essential for photoreceptor disc morphogenesis leads to failure of correct rod and cone outer segment formation in the Chx10(or-J/or-J PMID: 14744875
  11. linked to maintenance of the neural retina PMID: 15459106
  12. repression of Mitf by FGF is Chx10 dependent, indicating that FGF, Chx10 and Mitf are components of a pathway that determines and maintains the identity of the neuroretina PMID: 15576400
  13. CHX10 may target specific motifs to inhibit rod photoreceptor gene expression in bipolar cells PMID: 16236706
  14. Chx10 directly controls bipolar cell genesis by inhibiting rod differentiation independent of its temporally limited early effect on retinal progenitor cell proliferation PMID: 16547132
  15. Scan reveals significant loci on chromosomes 6 and 14, where the CASA/Rk alleles of Chx10 from a backcross between 129/SvJ and Mus musculus castaneus are maintained selectively and show a much milder Chx10 phenotype. PMID: 16783634
  16. Although Vsx1 mRNA is upregulated in Chx10/Vsx2 deficient RPCs, Vsx1 does not genetically compensate for loss of Chx10/Vsx2, demonstrating that Prd-L:CVC genes, although important, are not absolutely required to initiate retinal development. PMID: 17919464
  17. Vsx2/Chx10 ensures the correct timing and magnitude of Hedgehog signaling in the mouse retina. PMID: 18417110
  18. Pax6 interacts with Chx10 via its paired domain or homeodomain (HD) and the HD of Chx10, resulting in enhanced transactivation by Pax6. This interaction was modeled on a demonstrated interaction between zebrafish Pax6 and mouse Chx10. PMID: 11069920

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Involvement in disease
Defects in Vsx2 are the cause of ocular retardation (OR(J)), a mouse disease characterized by microphthalmia, progressive destruction of the retina, and absence of the optic nerve. The OR(J) mutation is due to an OCHR (STOP) mutation.
Subcellular Location
Nucleus.
Protein Families
Paired homeobox family
Tissue Specificity
Expressed in the retina (at protein level). Expressed throughout the anterior optic vesicle and all neuroblasts of the optic cup (at protein level). In the mature and postnatal retina is restricted to the inner nuclear layer, in which its expression decre
Database Links
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