Recombinant Mycoplasma pneumoniae Uncharacterized glycosyltransferase MG335.2 homolog (MPN_483)

Code CSB-YP301927MLW
MSDS
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Source Yeast
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Code CSB-EP301927MLW
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Source E.coli
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Code CSB-EP301927MLW-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP301927MLW
MSDS
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Source Baculovirus
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Code CSB-MP301927MLW
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MPN_483
Uniprot No.
Alternative Names
MPN_483; MP359; P01_orf341Processive diacylglycerol beta-glycosyltransferase; EC 2.4.1.-; Beta-monoglycosyldiacylglycerol synthase; Beta-MGS; MGlyDAG synthase; Diglycosyldiacylglycerol synthase; Beta-DGS; DGlyDAG synthase; Glycosyl-beta-1,6-galactosyldiacylglycerol synthase; UDP-galactose:1,2-diacylglycerol 3-beta-D-galactosyltransferase; UDP-glucose:1,2-diacylglycerol 3-beta-D-glucosyltransferase
Species
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Expression Region
1-341
Target Protein Sequence
MNKLISILVP CYQSQPFLDR FFKSLLKQDW NGVKVIFFND NKPDPTYEIL KQFQQAHPQL AIEVHCGEKN VGVGGSRDQL INYVDTPYFY FVDPDDEFSD PNCFKAIVET IQGENFDIAV LNSIVYLQML KNDFLIKHIP LKNIFQGKVK LNPDNTVNHL HYIQNNDQYI WNIVINTAFF KALDLQFVNR FIEDIAVWFP IMFKAQKVLW IDVNGVNYYL RPNSASTQKN SIKLLSFIEA YERLYFHLKK VGKLADFIDP NNKIESRFWR RQAFIWFSFI NVSWMKAEFE QTKSVLQKLF DFMEANGIYD RVFTNKHHGI YLLWVNRLKH FKKLVQAQPH L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Processive glycosyltransferase involved in the biosynthesis of both the non-bilayer-prone beta-monoglycosyldiacylglycerol and the bilayer-forming membrane lipid glucosyl-galactosyldiacylglycerol and digalactosyl-diacylglycerol. These components contribute to regulate the properties and stability of the membrane. Catalyzes sequentially the transfers of glucosyl or galactosyl residues from UDP-Glc or UDP-Gal to diacylglycerol (DAG) acceptor to form the corresponding beta-glycosyl-DAG (3-O-(beta-D-glycopyranosyl)-1,2-diacyl-sn-glycerol). Then, only beta-galactosyl-DAG (3-O-(beta-D-galactopyranosyl)-1,2-diacyl-sn-glycerol) can act as acceptor to give the beta-glycosyl-beta-galactosyl-DAG product (3-O-(beta-D-glycopyranosyl-(1->6)-D-galactopyranosyl)-1,2-diacyl-sn-glycerol). It can also use alpha-Gal-beta-Gal-DAG, ceramide (Cer) and beta-Gal-Cer as sugar acceptors. The enzyme is supposed to be mainly a galactosyltransferase, with higher glycosyltransferase activity for the addition of the second glycosyl on beta-Gal-DAG as acceptor. The main glycolipid produced in vivo is beta-Glc-beta-Gal-DAG with a beta-1,6 linkage.
Gene References into Functions
  1. One (MPN483) of the three predicted glycosyltransferases (GTs; all essential) was both processive and promiscuous, synthesizing most of the identified glycolipids. PMID: 17697098
Subcellular Location
Cell membrane.
Protein Families
Glycosyltransferase 2 family
Database Links

KEGG: mpn:MPN483

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