Recombinant Naja mossambica Phospholipase A2 isozyme CM-III

Code CSB-YP360423NAH
MSDS
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Source Yeast
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Code CSB-EP360423NAH
MSDS
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Source E.coli
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Code CSB-EP360423NAH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360423NAH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP360423NAH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
Basic phospholipase A2 CM-III; svPLA2; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase
Species
Naja mossambica (Mozambique spitting cobra)
Expression Region
1-118
Target Protein Sequence
NLYQFKNMIH CTVPSRPWWH FADYGCYCGR GGKGTPVDDL DRCCQVHDNC YEKAGKMGCW PYFTLYKYKC SQGKLTCSGG NSKCGAAVCN CDLVAANCFA GARYIDANYN INFKKRCQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Snake venom phospholipase A2 (PLA2) that shows several activities. It shows strong anticoagulant activity, probably by binding to coagulation factor Xa (F10) and inhibiting the formation of the prothrombinase complex, shows direct hemolytic action, causes neuromuscular blockade with a gradual contracture and a decreased sensitivity to ACh and KCl, abolishes twitches evoked by indirect stimulation earlier than those by direct stimulation (in the mouse phrenic nerve-diaphragm preparation), and causes myonecrosis when injected intramuscularly. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Subcellular Location
Secreted.
Protein Families
Phospholipase A2 family, Group I subfamily, D49 sub-subfamily
Tissue Specificity
Expressed by the venom gland.
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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