Recombinant Pig Pulmonary surfactant-associated protein D (SFTPD)

Code CSB-YP865048PI
MSDS
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Source Yeast
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Code CSB-EP865048PI
MSDS
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Source E.coli
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Code CSB-EP865048PI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865048PI
MSDS
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Source Baculovirus
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Code CSB-MP865048PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SFTPD
Uniprot No.
Alternative Names
SFTPD; Pulmonary surfactant-associated protein D; PSP-D; SP-D; Lung surfactant protein D
Species
Sus scrofa (Pig)
Expression Region
21-378
Target Protein Sequence
AEMKTYSQRA VANACALVMC SPMENGLPGR DGRDGREGPR GEKGDPGLPG AVGRAGMPGL AGPVGPKGDN GSTGEPGAKG DIGPCGPPGP PGIPGPAGKE GPSGQQGNIG PPGTPGPKGE TGPKGEVGAL GMQGSTGARG PAGLKGERGA PGERGAPGSA GAAGPAGATG PQGPSGARGP PGLKGDRGPP GERGAKGESG LPGITALRQQ VETLQGQVQR LQKAFSQYKK VELFPNGRGV GEKIFKTGGF EKTFQDAQQV CTQAGGQMAS PRSETENEAL SQLVTAQNKA AFLSMTDIKT EGNFTYPTGE PLVYANWAPG EPNNNGGSSG AENCVEIFPN GKWNDKACGE LRLVICEF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Gene References into Functions
  1. The authors demonstrated that the number and position of glycosylation sites determine viral susceptibility to the neutralizing activity of porcine SP-D. PMID: 22921759
  2. Data show that interactions of surfactant protein D (pSP-D) with influenza A virus mediated by the N-linked carbohydrate moiety in pSP-D depend on the terminal sialic acids present on this moiety. PMID: 14672916
  3. The presence of SP-D in endothelial cells which is NO-, PI(3)K/Akt- and Erk-dependent is demonstrated. It suggests a protective role of SP-D in these cells. PMID: 19010548
  4. The importance of SP-D in innate immunity is underlined by its expression at the potential ports of entry of pathogens: lung, tongue, intestinal tract, thymus, skin, gall bladder, lacrimal gland, esophagus, stomach, kidney, liver, prostate and spleen. PMID: 17420052
Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Protein Families
SFTPD family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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