Recombinant Pseudomonas putida Siroheme synthase (cysG)

Code CSB-YP406260PZN
MSDS
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Source Yeast
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Code CSB-EP406260PZN
MSDS
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Source E.coli
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Code CSB-EP406260PZN-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP406260PZN
MSDS
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Source Baculovirus
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Code CSB-MP406260PZN
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cysG
Uniprot No.
Alternative Names
cysG; Pput_1834Siroheme synthase [Includes: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; EC 2.1.1.107; SUMT; Uroporphyrinogen III methylase; UROM); Precorrin-2 dehydrogenase; EC 1.3.1.76); Sirohydrochlorin ferrochelatase; EC 4.99.1.4)]
Species
Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Expression Region
1-463
Target Protein Sequence
MDYLPLFHKL QGGRVLVVGG GEIALRKARL LADAGGVLRV VAPDVDGQLA ALAREGGGEV LVRGYQAADL VGCRLVIAAT DDPGLNAQVS ADAQALSVPV NVVDAPALCT VIFPAIVDRS PLVIAVSSGG DAPVLARLIR AKLEAWIPSA YGELAGLAAR FRHKVKSLYP DVNQRRGFWE TVFQGPIAER QLAGQGAEAE RLLQAMVDGA PVQQGGEVYL VGAGPGDPDL LTFRALRLMQ QADVVLYDRL VAPAIIDMCR RDAERIYVGK RRADHSVPQD QINRLLVDLA RQGKRVLRLK GGDPFIFGRG GEEIEELAEH GIPFQVVPGI TAASGCSAYG GIPLTHRDYA QSVRFVTGHL KDGTSNLPWT DLVAPAQTLV FYMGLVGLPT ICAELIRHGR AASTPAALVQ QGTTRNQRVF TGTLADLPDL VAQHEVHAPT LVIVGEVVQL RDKLAWFEGS QNS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.
Protein Families
Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family; Precorrin methyltransferase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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