Recombinant Ralstonia solanacearum HTH-type transcriptional regulator phcA (phcA)

Code CSB-YP345625RAR
MSDS
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Source Yeast
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Code CSB-EP345625RAR
MSDS
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Source E.coli
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Code CSB-EP345625RAR-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345625RAR
MSDS
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Source Baculovirus
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Code CSB-MP345625RAR
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
phcA
Uniprot No.
Alternative Names
phcA; RSc2748; RS00106; HTH-type transcriptional regulator PhcA; Virulence genes transcriptional regulator PhcA
Species
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Expression Region
1-347
Target Protein Sequence
MVNVDTKLLV IFVELLSKRN ATYVAEKMHM TAPAVSHSLG RLREIFDDPL FIRVPHGLTP TPKALELGPK VREMLDLWAA INEGDIATFD PLEAAGTFNI SFAGTLGDAL FDRFLLRVKR LAPGLQVRLT ESSSWEADVA AMRSNELDLA FSPFPTRHPE IVEEVVTSFN MWVCARKDHP VLKDHCSLDQ YLECEHIFIA QGNPGTRAAP SLIPLDYALQ QRGLKRHSTM TVHAWRTQAE VAAQTDMIFT VNSLMKDLVC ETYNLNAFPL PSELETVLGL NMLWHRSRNT HPMLVWARNL FKQVVAEYTG KASIAPMHPP MLTDDSGKSG KTGKGDAEKE DESRLSV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulates the transcription of one or more of the genes involved in virulence.
Gene References into Functions
  1. The PhcA regulon identified is the largest regulon described to date in the Ralstonia solanacearum species complex with 1581 regulated genes, representing about 30% of the bacterial genome. PMID: 29408557
  2. Ralstonia solanacearum iron scavenging by the siderophore staphyloferrin B is controlled by PhcA. PMID: 15547261
  3. provide genetic evidence that PhcA also represses the expression of hrp genes that code for the Type III protein secretion system, a major pathogenicity determinant in this bacterium PMID: 15811321
Database Links

KEGG: rso:RSc2748

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