Recombinant Rat Acetoacetyl-CoA synthetase (Aacs)

Code CSB-YP001012RA
MSDS
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Source Yeast
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Code CSB-EP001012RA
MSDS
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Source E.coli
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Code CSB-EP001012RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001012RA
MSDS
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Source Baculovirus
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Code CSB-MP001012RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Aacs
Uniprot No.
Alternative Names
AacsAcetoacetyl-CoA synthetase; EC 6.2.1.16
Species
Rattus norvegicus (Rat)
Expression Region
1-672
Target Protein Sequence
MSKLARLERE EIMECQVMWE PDSKKDTQMD RFRAAVGTAC GLALGNYDDL YHWSVRSYSD FWAEFWKFSG IVCSRMYDEV VDTSKGIADV PEWFRGSRLN YAENLLRHKE NDRVALYVAR EGREEIAKVT FEELRQQVAL FAAAMRKMGV KKGDRVVGYL PNSAHAVEAM LAAASIGAIW SSTSPDFGVN GVLDRFSQIQ PKLIFSVEAV VYNGKEHGHL EKLQRVVKGL PDLQRVVLIP YVLPREKIDI SKIPNSMFLD DFLASGTGAQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM VHSAGGTLIQ HLKEHVLHGN MTSSDILLYY TTVGWMMWNW MVSALATGAS LVLYDGSPLV PTPNVLWDLV DRIGITILGT GAKWLSVLEE KDMKPMETHN LHTLHTILST GSPLKAQSYE YVYRCIKSTV LLGSISGGTD IISCFMGQNS SIPVYKGEIQ ARNLGMAVEA WDEEGKTVWG ASGELVCTKP IPCQPTHFWN DENGSKYRKA YFSKYPGVWA HGDYCRINPK TGGIVMLGRS DGTLNPNGVR FGSSEIYNIV EAFDEVEDSL CVPQYNRDGE ERVVLFLKMA SGHTFQPDLV KHIRDAIRLG LSARHVPSLI LETQGIPYTI NGKKVEVAVK QVIAGKTVEH RGAFSNPESL DLYRDIPELQ DF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.
Gene References into Functions
  1. Prenatal nicotine exposure can cause DNA hypermethylation of the AACS promoter in the rat fetal adrenal. PMID: 26776438
  2. AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development PMID: 16055091
  3. As acetoacetyl-CoA synthetase was expressed in neural-like cells, ketone bodies are assumed to be utilized for the synthesis of lipidic substances and to cause metabolic disorders in the nervous system of genetically obese rats. PMID: 19219059
Subcellular Location
Cytoplasm, cytosol.
Protein Families
ATP-dependent AMP-binding enzyme family
Tissue Specificity
Abundant in male subcutaneous white adipose tissue after weaning. In white adipose tissue, it is preferentially detected in mature adipocytes but not in preadipocytes. The expression in primary preadipocytes increases during the adipocyte differentiation.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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