Recombinant Rat Alpha-mannosidase 2 (Man2a1)

Code CSB-YP013387RA
MSDS
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Source Yeast
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Code CSB-EP013387RA
MSDS
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Source E.coli
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Code CSB-EP013387RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013387RA
MSDS
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Source Baculovirus
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Code CSB-MP013387RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Man2a1
Uniprot No.
Alternative Names
Man2a1; Mana2; Alpha-mannosidase 2; EC 3.2.1.114; Golgi alpha-mannosidase II; AMan II; Man II; Mannosidase alpha class 2A member 1; Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Species
Rattus norvegicus (Rat)
Target Protein Sequence
GFSPHIIRVE RKGQLSILQE KIDHLERLLA ENNEIISNIR DSVINLSESV EDGPRGPAGN ASQGSAHLHS AQLALQADPK DCLFASQSGN QHRDVQMLDV YDLIPFDNPD GGVWKQGFDI KYEADEWDRE PLQVFVVPHS HNDPGWLKTF NDYFRDKTQY IFNNMVLKLK EDSSRKFIWS EISYLAKWWD IIDNPKKEAV KSLLQNGQLE IVTGGWVMAD EATTHYFALI DQLIEGHQWL EKNLGVKPRS GWAIDPFGHS PTMTYLLKRA GFSHMLIQRV HYSVKKHFSL QKTLEFFWRQ NWDLGSTTDI LCHMMPFYSY DIPHTCGPDP KICCQFDFKR LPGGRYGCPW GVPPEAISPG NVQSRAQMLL DQYRKKSKLF RTKVLLAPLG DDFRFSEYTE WDLQYRNYEQ LFSYMNSQPH LKVKIQFGTL SDYFDALEKS VAAEKKGGQS VFPALSGDFF TYADRDDHYW SGYFTSRPFY KRMDRIMEF
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Gene References into Functions
  1. Data suggest that hepatocyte expression of Man2a1 (Golgi apparatus alpha-mannosidase) and Man2b1 (lysosome alpha-mannosidase) is down-regulated by biological toxin swainsonine as part of locoweed/Oxytropis kansuensis hepatotoxic damage. PMID: 25797317
  2. swainsonine from Oxytropis kansuensis could reduce the expression of MAN2A1 in brain tissues PMID: 24741992
  3. Man2a1 expression increases at night ~10-fold in the rat pineal gland, by a mechanism involving neural stimulation and norepinephrine - cyclic AMP control. PMID: 19103603
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyl hydrolase 38 family
Tissue Specificity
Liver.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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