Recombinant Rat Double C2-like domain-containing protein beta (Doc2b)

Code CSB-YP007092RA
MSDS
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Source Yeast
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Code CSB-EP007092RA
MSDS
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Source E.coli
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Code CSB-EP007092RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007092RA
MSDS
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Source Baculovirus
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Code CSB-MP007092RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Doc2b
Uniprot No.
Alternative Names
Doc2bDouble C2-like domain-containing protein beta; Doc2-beta
Species
Rattus norvegicus (Rat)
Expression Region
1-412
Target Protein Sequence
MTLRRRGEKA TISIQEHMAI DVCPGPIRPI KQISDYFPRF PRGLPPTAAP RASAPPDAPA RSPAATAGPR SPSDGARDDD EDVDQLFGAY GASPGPSPGP SPVRPPAKPP EDEPDADGYE SDDCTALGTL DFSLLYDQEN NALHCTISKA KGLKPMDHNG LADPYVKLHL LPGASKANKL RTKTLRNTLN PSWNETLTYY GITDEDMIRK TLRISVCDED KFRHNEFIGE TRVPLKKLKP NHTKTFSICL EKQLPVDKAE DKSLEERGRI LISLKYSSQK QGLLVGIVRC AHLAAMDANG YSDPYVKTYL KPDVDKKSKH KTAVKKKTLN PEFNEEFCYE IKHGDLAKKT LEVTVWDYDI GKSNDFIGGV VLGINAKGER LKHWFDCLKN KDKRIERWHT LTNEIPGAVL SD
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.
Gene References into Functions
  1. acts directly on membranes and stabilizes hemifusion intermediate in cell-free system PMID: 26395669
  2. these data reveal the key determinants of Doc2beta that underlie its function during the slow phase of synaptic transmission. PMID: 26195798
  3. Mutations that disrupt Ca(2)-binding activity endow Doc2beta with novel functional properties during synaptic transmission. PMID: 24356452
  4. Doc2b exocytotic functions appear to be distinct from how synaptotagmin-1 promotes synaptic neurotransmitter release. PMID: 23427263
Subcellular Location
Cytoplasm. Cytoplasmic granule. Cell membrane; Peripheral membrane protein. Note=Translocates to the plasma membrane in a calcium-dependent manner.
Tissue Specificity
Expressed in brain; highly enriched in neurons.
Database Links

KEGG: rno:81820

UniGene: Rn.10689

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