Recombinant Rat Eukaryotic translation initiation factor 2-alpha kinase 3(Eif2ak3) ,partial

Code CSB-YP007512RA
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Source Yeast
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Code CSB-EP007512RA
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Source E.coli
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Code CSB-EP007512RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007512RA
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Source Baculovirus
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Code CSB-MP007512RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Eif2ak3
Uniprot No. Q9Z1Z1
Alternative Names Eif2ak3; Pek; PerkEukaryotic translation initiation factor 2-alpha kinase 3; EC 2.7.11.1; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase
Species Rattus norvegicus (Rat)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Gene References into Functions
  1. These results suggest that the administration of Pien Tze Huang ameliorated the severity of alcoholic and high-fat diet rats possibly by inhibiting the Hcy-induced PERK/eIF2alpha pathway. PMID: 30005895
  2. AS-IV may attenuate inflammatory responses by disrupting the crosstalk between autophagy and the PERK-eIF2alpha pathway and may be an ideal agent for treating inflammatory pulmonary diseases. PMID: 28641278
  3. Pre-ischemia melatonin treatment alleviated acute neuronal injury after ischemic stroke by inhibiting endoplasmic reticulum stress-dependent autophagy via PERK and IRE1 signalings PMID: 28178380
  4. these results confirmed that ER stress-mediated apoptosis contributes to the protection effects of naringenin against H/R injury, which is potentially involved in ATF6, IRE1alpha and PERK signaling activation. PMID: 27785700
  5. Cirrhotic rats with diabetes showed higher intrahepatic ET-1 vasoresponsiveness than normoglycemic cirrhotic rats. This effect may be related, at least in part, to intrahepatic ETA R receptor and pERK over-expression. PMID: 24636620
  6. Suggest role for PERK in modulating fluoride induced bone formation and bone resorption. PMID: 25132241
  7. PERK was activated in reactive oxygen species-mediated endoplasmic reticulum stress-induced apoptosis in a rat model of diabetic cardiomyopathy. PERK deletion in myocytes showed a protective effect against apoptosis induced by high-glucose incubation. PMID: 24180212
  8. Autophagy induced by (125)I radiation was involved in endoplasmic reticulum stress and mainly dependent on PERK-eIF2alpha pathway. PMID: 24223705
  9. interplay between calcineurin and PERK regulates beta-cell Ca(2+) signaling and insulin secretion, and that loss of this interaction may have profound implications in insulin secretion defects associated with diabetes. PMID: 24114838
  10. The number of PERK- and CHOP-positive cells is significantly higher in diabetic and high glucose-exposed retina cultures than in normal retinas. PMID: 21767604
  11. Activation of the PERK/eIF2a signaling pathway contributes to the occurrence and development of hepatocyte apoptosis in alcoholic liver injury. PMID: 21059295
  12. eukaryotic translation initiation factor 2 alpha kinase 3 (PERK) is activated following oxygen-glucose deprivation of rat cortical cells PMID: 21525936
  13. A decrease in the ATP/energy status in response to a decrease in glucose results in SERCA pump inhibition, the efflux of Ca2+ from the ER, and the activation of PERK, which plays an important role in both pancreatic beta-cell function and survival. PMID: 21193559
  14. Endoplasmic reticulum stress blunts cytokine-triggered activation of NF-kappaB, in part through PERK- and IRE1-mediated preferential induction of C/EBPbeta. PMID: 19875812
  15. Translation mediated by the internal ribosome entry site of the cat-1 mRNA is regulated by glucose availability in a PERK kinase-dependent manner PMID: 11781318
  16. Renal ischemia and reperfusion activates the eIF 2 alpha kinase PERK. PMID: 15936177
  17. force-induced apoptosis depends on a novel function of PERK that occurs in addition to its canonical role in the unfolded protein response PMID: 18550523
  18. Dysregulation of endoplasmic reticulum chaperone/folding enzymes ERp72 and GRP78/BiP occurred early after ablation of PERK function PMID: 19732428

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Subcellular Location Endoplasmic reticulum membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
Tissue Specificity Ubiquitous.
Database Links

KEGG: rno:29702

STRING: 10116.ENSRNOP00000008451

UniGene: Rn.24897

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