Recombinant Rat High affinity choline transporter 1 (Slc5a7), partial

Code CSB-YP881356RA
MSDS
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Source Yeast
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Code CSB-EP881356RA
MSDS
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Source E.coli
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Code CSB-EP881356RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881356RA
MSDS
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Source Baculovirus
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Code CSB-MP881356RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slc5a7
Uniprot No.
Alternative Names
Slc5a7; Cht1; High affinity choline transporter 1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transmembrane transporter that imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.
Gene References into Functions
  1. Young and old cardiac myocytes are able to synthesize, transport and excrete acetylcholine in the heart. Adult cardiomyocytes express the high affinity choline transporter 1 (CHT-1). PMID: 20510655
  2. ultrastructural distribution of CHT in anteroventral thalamus and ventral tegmental area; immunoreactivity for this protein was predominantly localized to synaptic vesicles and minimally to the plasma membrane of axons in both regions PMID: 20394050
  3. CHT1 plays a key role in acetylcholine synthesis in NIH3T3ChAT 112-1 cells which stably expresses both rat CHT1 and mouse ChAT. PMID: 19405101
  4. high-affinity choline transporter, choline transporter 1, in nerve fibers and epithelial cells in the human and rat skin supporting the pivotal role of this transporter in both the neuronal and non-neuronal cholinergic system of the skin. PMID: 12406342
  5. close apposition of CHT1 to reported sites of localization of choline acetyltransferase in these cells is strongly in favour of ACh synthesis being fuelled by choline uptake via CHT1 in these epithelia PMID: 12628461
  6. This protein is expressed in the rat trachea. PMID: 12654636
  7. 18.0 mg/kg dose of chlorpyrifos was associated with (brain region-dependent) decreases in nerve growth factor receptors and cholinergic proteins including the high-affinity choline transporter PMID: 17548533
  8. findings show here that internalized CHT1 is rapidly recycled back to the cell surface PMID: 18088276

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Subcellular Location
Membrane; Multi-pass membrane protein. Cell membrane. Cell junction, synapse.
Protein Families
Sodium:solute symporter (SSF) (TC 2.A.21) family
Tissue Specificity
Expressed in basal forebrain, brain stem, spinal chord, and striatum. Specific for cholinergic neurons.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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