Recombinant Rat Homeobox protein Nkx-6.1 (Nkx6-1)

Code CSB-YP015850RA
MSDS
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Source Yeast
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Code CSB-EP015850RA
MSDS
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Source E.coli
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Code CSB-EP015850RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015850RA
MSDS
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Source Baculovirus
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Code CSB-MP015850RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nkx6-1
Uniprot No.
Alternative Names
Nkx6-1; Nkx6.1; Nkx6a; Homeobox protein Nkx-6.1; Homeobox protein NK-6 homolog A
Species
Rattus norvegicus (Rat)
Expression Region
1-365
Target Protein Sequence
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS SSSSSSSSSP SPPLGAHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASATSASAAA AAAAAAAAAA ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP SAAAVAAVGR YPKPLAELPG RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT RPTFSGQQIF ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS GGSLLLHASE AEGSS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.
Gene References into Functions
  1. These data demonstrate that c-Fos is critical for Nkx6.1-mediated expansion of functional beta-cell mass. PMID: 27164028
  2. The homeobox beta-cell transcription factor Nkx6.1 induces beta-cell proliferation by activating the orphan nuclear receptors Nr4a1 and Nr4a3. PMID: 26030060
  3. Nkx6.1 plays a vital role in astrocyte specification and differentiation in the ventral spinal cord. PMID: 25285789
  4. K cells could be reprogrammed partially to beta-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture. PMID: 24365150
  5. Beta cell maturation accompanied by increased IL-1beta sensitivity is, in part, dependent upon the expression of genes regulated by Nkx6.1 (Nkx6.1) PMID: 15605246
  6. Nkx6.1 homeodomain transcription factor suppresses glucagon expression and regulates glucose-stimulated insulin secretion in islet beta cells. PMID: 15883383
  7. Overexpression of Nkx6.1 in islets caused a clear enhancement of glucose-stimulated insulin secretion. PMID: 18347054

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Subcellular Location
Nucleus.
Tissue Specificity
Pancreatic beta cells.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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