Recombinant Rat Hsc70-interacting protein (St13)

Code CSB-YP022744RA
MSDS
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Source Yeast
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Code CSB-EP022744RA
MSDS
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Source E.coli
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Code CSB-EP022744RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022744RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP022744RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
St13
Uniprot No.
Alternative Names
St13; Fam10a1; HipHsc70-interacting protein; Hip; Protein FAM10A1; Protein ST13 homolog
Species
Rattus norvegicus (Rat)
Expression Region
1-368
Target Protein Sequence
MDPRKVSELR AFVKMCRQDP SVLHTEEMRF LREWVESMGG KVPPATHKAK SEENTKEEKR DKTTEDNIKT EEPSSEESDL EIDNEGVIEA DTDAPQEMGD ENAEITEAMM DEANEKKGAA IDALNDGELQ KAIDLFTDAI KLNPRLAILY AKRASVFVKL QKPNAAIRDC DRAIEINPDS AQPYKWRGKA HRLLGHWEEA ARDLALACKL DYDEDASAML REVQPRAQKI AEHRRKYERK REEREIKERI ERVKKAREEH EKAQREEEAR RQSGSQFGSF PGGFPGGMPG NFPGGMPGMG GAMPGMAGMP GLNEILSDPE VLAAMQDPEV MVAFQDVAQN PSNMSKYQNN PKVMNLISKL SAKFGGHS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.
Gene References into Functions
  1. The tetratricopeptide repeat domain of rat Hip with the Hsp70 ATPase domain through an extensive interface, to form a bracket that locks ADP in the binding cleft. PMID: 23812373
  2. The co-aggregation phenomenon of heat shock protein 70 with alpha-synuclein can be prevented in vitro by co-chaperone Hip (ST13), which can facilitate depletion of Hsc70 by amyloidogenic polypeptides, impairing chaperone proteostasis. PMID: 19875982
  3. HIP was cleaved by granzyme B; the granzyme B-mediated cleavage site was mapped to the BID loop-like region of HIP; data implicate HIP in the proteolytic cascade of some apoptotic pathways PMID: 17013759
Subcellular Location
Cytoplasm.
Protein Families
FAM10 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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