Product Details

Purity

>85% (SDS-PAGE)

Target Names

Lrp2

Uniprot No.

P98158

Alternative Names

Lrp2; Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 330; gp330; Megalin

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Multiligand endocytic receptor. Acts together with CUBN to mediate endocytosis of high-density lipoproteins. Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B. In the kidney, mediates the tubular uptake and clearance of leptin. Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium. Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight. Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells. Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP. Mediates renal uptake of metallothionein-bound heavy metals. Together with CUBN, mediates renal reabsorption of myoglobin. Mediates renal uptake and subsequent lysosomal degradation of APOM. Plays a role in kidney selenium homeostasis by mediating renal endocytosis of selenoprotein SEPP1. Mediates renal uptake of the antiapoptotic protein BIRC5/survivin which may be important for functional integrity of the kidney. Mediates renal uptake of matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1. Mediates endocytosis of Sonic hedgehog protein N-product (ShhN), the active product of SHH. Also mediates ShhN transcytosis. In the embryonic neuroepithelium, mediates endocytic uptake and degradation of BMP4, is required for correct SHH localization in the ventral neural tube and plays a role in patterning of the ventral telencephalon. Required at the onset of neurulation to sequester SHH on the apical surface of neuroepithelial cells of the rostral diencephalon ventral midline and to control PTCH1-dependent uptake and intracellular trafficking of SHH. During neurulation, required in neuroepithelial cells for uptake of folate bound to the folate receptor FOLR1 which is necessary for neural tube closure. In the adult brain, negatively regulates BMP signaling in the subependymal zone which enables neurogenesis to proceed. In astrocytes, mediates endocytosis of ALB which is required for the synthesis of the neurotrophic factor oleic acid. Involved in neurite branching. During optic nerve development, required for SHH-mediated migration and proliferation of oligodendrocyte precursor cells. Mediates endocytic uptake and clearance of SHH in the retinal margin which protects retinal progenitor cells from mitogenic stimuli and keeps them quiescent. Plays a role in reproductive organ development by mediating uptake in reproductive tissues of androgen and estrogen bound to the sex hormone binding protein SHBG. Mediates endocytosis of angiotensin-2. Also mediates endocytosis of angiotensin 1-7. Binds to the complex composed of beta-amyloid protein 40 and CLU/APOJ and mediates its endocytosis and lysosomal degradation. Required for embryonic heart development. Required for normal hearing, possibly through interaction with estrogen in the inner ear.

Gene References into Functions

Data suggest that, in acute kidney injury (here, due to cadmium poisoning), kidney exhibits down-regulation of megalin receptor activity; up-regulation of NFkappaB, TLR2 (toll like receptor-2), and TLR4 (toll like receptor-4); and significant reduction in GSH (glutathione). PMID: 29286200
Exocytosis-mediated urinary C-megalin excretion is associated with the development and progression of diabetic nephropathy in T2DM, particularly due to megalin-mediated lysosomal dysfunction in proximal tubules. PMID: 28289043
Data suggest both LRP1 and LRP2 are necessary in growth cones for ligand- (zinc metallothionein IIa-)mediated chemotactic calcium-dependent signal transduction in sensory neurons, for axon guidance/neurogenesis, and for peripheral nerve regeneration. PMID: 26598525
Megalin-mediated B12 endocytosis in renal tubules may be regulated by methylmalonic acid. PMID: 26248135
The inhibition of megalin/cubilin or lysosomal cathepsin B reduced albuminuria-induced NLRP3 inflammasome activation. PMID: 26025362
ARH knockdown leads to fast recycling of megalin via a Rab35 pathway. PMID: 23836931
Dietary resistant starch normalized megalin and Dab2 mRNA overexpression in diabetic rats. PMID: 23677864
megalin-dependent endocytosis has a role along the proximal nephron in the intrarenal renin angiotensin system PMID: 20966072
Amino acids 107-136 of megalin-CT containing the second NPXY-like motif are critical for apical sorting and targeting, whereas the regions containing the first and third NPXY motifs are required for efficient endocytosis. PMID: 12519751
megalin functions for endocytosis of AGE via an indirect mechanism. PMID: 12707383
endocytic receptors megalin and cubilin are involved in renal reabsorption of myoglobin PMID: 12724130
Because thyroglobulin binding to megalin is greatest at acidic pH, it is possible that TG does not dissociate from megalin in the lysosomal pathway. However, the pH-dependence of TG binding to megalin does not account for much of transcytosis. PMID: 12809172
Megalin is an endocytic receptor that binds multiple ligands and is essential for many physiological processes such as brain development PMID: 14528014
megalin has a role in thyroid homeostasis with possible implications in thyroid diseases PMID: 14657389
Evidence is provided in a rat model that a megalin N-terminal domain includes B and T cell epitopes sufficient for a pathogenic autoimmune response and that a native-like conformation and glycosylation are essential for induction of Heymann nephritis. PMID: 14764706
the hinge SCKKSCC region of metallothionein as a critical site for megalin(lrp2) binding PMID: 15126248
These data suggest that light chains bind the tandem megalin-cubilin complex. PMID: 15286052
Cubilin expression at later developmental stages of the rodent embryo and its correlation to that of megalin was investigated. PMID: 16027047
Megalin mediates IGF-I-induced clearance of Abeta and is involved in IGF-I transport into brain. Attenuated IGF-I/megalin input may contribute to increased risk of neurodegeneration, including late-onset Alzheimer's disease. PMID: 16306401
In nephrotic rats, the rate of uptake of albumin by the proximal tubule (PT) is decreased. This is consistent with reduced expression of clathrin, megalin, and vacuolar H(+)-ATPase A subunit, proteins that are critical components of PT endocytosis. PMID: 17228368
The solution structure of the twelfth cysteine-rich ligand-binding repeat with class A motif found in megalin features two short beta-strands and two helical turns. PMID: 17245526
The present data indicate that leptin represents a novel megalin ligand of importance in the levels and therapeutic actions of leptin into the brain. PMID: 17324488
Significant amounts of megalin and cubilin were present in the glomerular cells in addition to the proximal tubular cells. The quantity of these receptors progressively increased in the glomerulus with age. PMID: 17453355
Upregulation of B7.1 with the downregulation of GLEPP1, Wilms' tumor gene (WT1), megalin, and vascular endothelial growth factor started early and persisted through the course of primary glomerular disease PMID: 17457373
Three distinct regions of cubilin bind to megalin and its interactions with both megalin and amnionless are essential for its intracellular stability. PMID: 17990981
Astrocytes express megalin, an endocytic receptor for multiple ligands including albumin; megalin is required for albumin binding and internalization in the astrocyte. PMID: 18466341
There is AT(1A)R- and insulin-mediated competitive signaling cross talk to regulate megalin expression in cultured proximal tubule cells. PMID: 18927221
cubilin and megalin constitute a dual-receptor complex facilitating the function of each other in the inner ear PMID: 19202329

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Subcellular Location

Apical cell membrane; Single-pass type I membrane protein. Endosome lumen. Membrane, clathrin-coated pit. Cell projection, dendrite. Cell projection, axon.

Protein Families

LDLR family

Tissue Specificity

In the inner ear, expressed in the lumen of the endolymphatic sac where it localizes to macrophage-like cells as well as to mitochondria-rich and ribosome-rich epithelial cells (at protein level). In the inner ear, expressed in marginal cells of the stria

Database Links

KEGG: rno:29216

STRING: 10116.ENSRNOP00000066394

UniGene: Rn.26430

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Recombinant Rat Low-density lipoprotein receptor-related protein 2 (Lrp2), partial

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