Recombinant Rat Neurabin-1 (Ppp1r9a), partial

Code CSB-YP018557RA
MSDS
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Source Yeast
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Code CSB-EP018557RA
MSDS
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Source E.coli
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Code CSB-EP018557RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018557RA
MSDS
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Source Baculovirus
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Code CSB-MP018557RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ppp1r9a
Uniprot No.
Alternative Names
Ppp1r9a; Neurabin-1; Neurabin-I; Neural tissue-specific F-actin-binding protein I; PP1bp175; Protein phosphatase 1 regulatory subunit 9A; p180
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May be involved in neurite formation. Inhibits protein phosphatase 1-alpha activity. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction.
Gene References into Functions
  1. Data show that the PP1:spinophilin holoenzyme is dynamic in solution, which allows for an increased capture radius of spinophilin and is likely important for its biological role. PMID: 21094159
  2. The spinophilin interactome was charaterized in subcellular fractions solubilized from adult rodent striatum. PMID: 20124353
  3. recruit specific isoforms of protein phosphatase-1 catalytic subunits PMID: 12016225
  4. N-terminal F-actin-binding domain dictated neurabin I localization at actin cytoskeleton and promoted disassembly of stress fibers (neurabin I) PMID: 12052877
  5. there is a novel PP1gamma1-selective interaction domain in neurabin that may allow for selective regulation and/or subcellular targeting of PP1 isoforms PMID: 15016827
  6. Postsynaptic actin-bound neurabin-I-PP1 complex regulates synaptic transmission and bidirectional changes in hippocampal plasticity. PMID: 16899074
  7. NrbI-targeted PP1, in response to distinct synaptic activities, regulates the synaptic trafficking of specific AMPAR subunits PMID: 17460080
  8. study determined the structure of the neurabin SAM domain by solution state nuclear magnetic resonance spectroscopy PMID: 17600833
  9. Mutation of the Cdk5 phosphorylation site reduces the phenotypic consequences of Neurabin-I overexpression both in vitro and in vivo PMID: 17699587
  10. selective targeting of PP1gamma1 to F-actin by neurabins 1& 2 in intact cells requires both the canonical PP1-binding motif and an auxiliary PP1gamma1-selectivity determinant PMID: 18216290

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Subcellular Location
Cytoplasm, cytoskeleton. Cell junction, synapse, synaptosome.
Tissue Specificity
Brain, and widely expressed in neural tissue. Highly concentrated in synapses of developed neurons. In developing neurons, concentrated in the lamellipodia of the growth cone.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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