Recombinant Rat Neuronal regeneration-related protein (Nrep)

Code CSB-YP770500RA
MSDS
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Source Yeast
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Code CSB-EP770500RA
MSDS
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Source E.coli
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Code CSB-EP770500RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP770500RA
MSDS
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Source Baculovirus
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Code CSB-MP770500RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nrep
Uniprot No.
Alternative Names
Nrep; P311; Neuronal regeneration-related protein; Neuronal protein 3.1; Protein p311
Species
Rattus norvegicus (Rat)
Expression Region
1-68
Target Protein Sequence
MVYYPELLVW VSQEPFPYKE MEGGLTKGRL PVPKEVNRKK MDEPIAASLI PPGNHEPGSP AIGYLHPF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May have roles in neural function. Ectopic expression promotes axonal regeneration. Augments also motility of gliomas. May also have roles in cellular differentiation. Induces differentiation of fibroblast into myofibroblast and myofibroblast ameboid migration. Increases retinoic-acid regulation of lipid-droplet biogenesis. Down-regulates the expression of TGFB1 and TGFB2 but not of TGFB3. May play a role in the regulation of alveolar generation.
Gene References into Functions
  1. P311 may be involved in the pathogenesis of renal fibrosis by blocking TGF-beta1-mediated epithelial-mesenchymal transition via TGF-beta1-Smad-ILK pathway in unilateral ureteral occlusion kidneys. PMID: 29436600
  2. efficiently blocked Smad-ILK pathway activation and attenuated epithelial-mesenchymal transition induced by TGF-beta1 PMID: 26559022
  3. P311 gene, together with alpha-SMA and TGF-beta1 genes, was induced in the process of Tubular epithelial-myofibroblast transition (TEMT) in rat. PMID: 26335191
  4. identified a gene, previously designated P311, up-regulated in axotomized facial motoneurons; ectopically expressed P311 localizes in the cytoplasm and the nucleus; over-expression of P311 induces p21(WAF1/Cip1), leading PC12 cells to differentiate [P311] PMID: 15485502
Subcellular Location
Cytoplasm.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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