Recombinant Rat Selenocysteine insertion sequence-binding protein 2 (Secisbp2), partial

Code CSB-YP874102RA
MSDS
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Source Yeast
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Code CSB-EP874102RA
MSDS
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Source E.coli
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Code CSB-EP874102RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874102RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP874102RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Secisbp2
Uniprot No.
Alternative Names
Secisbp2; Sbp2; Selenocysteine insertion sequence-binding protein 2; SECIS-binding protein 2
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB.
Gene References into Functions
  1. Data show that a purified recombinant protein encompassing amino acids 517-777 of SECIS-binding protein 2 (SBP2) binds to SECIS elements with high affinity and selectivity. PMID: 25692238
  2. SBP2/SECIS complex activates eEFSec by directing functional interactions between Domain IV and the ribosome to promote Sec-tRNA(Sec) binding and accommodation into the ribosomal A-site PMID: 22992746
  3. The L7Ae RNA binding motif is a multifunctional domain required for the ribosome-dependent Sec incorporation activity of Secisbp2. PMID: 17636016
  4. the differential affinity of SBP2 for various SECIS elements will determine the expression pattern of the selenoproteome PMID: 17901054
  5. CUG-BP1 and HuR act as factors that bind to the SBP2 3' UTR, which suggests that TTR-RBPs play a role in the regulation of SBP2 PMID: 19106619
  6. ca. 70% of the SBP2 sequence is disordered, whereas the RNA binding domain appears to be folded and functional. PMID: 19467292
  7. The interaction of eIF4a3 with selenoprotein mRNA prevents the binding of SECIS binding protein 2, which is required for selenocysteine insertion, thereby inhibiting selenoprotein synthesis. PMID: 19716792

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Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous.
Database Links
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