Product Details

Purity

>85% (SDS-PAGE)

Target Names

Sik1

Uniprot No.

Q9R1U5

Alternative Names

Sik1; Kid2; Sik; Snf1lk; Serine/threonine-protein kinase SIK1; EC 2.7.11.1; Protein kinase KID2; Salt-inducible kinase 1; SIK-1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK

Species

Rattus norvegicus (Rat)

Expression Region

1-776

Target Protein Sequence

MVIMSEFSAV PTGTGQGQQK PLRVGFYDVE RTLGKGNFAV VKLARHRVTK TQVAIKIIDK TRLDSSNLEK IYREVQLMKL LNHPNIIKLY QVMETKDMLY IVTEFAKNGE MFDYLTSNGH LSENEARKKF WQILSAVEYC HNHHIVHRDL KTENLLLDGN MDIKLADFGF GNFYKPGEPL STWCGSPPYA APEVFEGKEY EGPQLDIWSL GVVLYVLVCG SLPFDGPNLP TLRQRVLEGR FRIPFFMSQD CETLIRRMLV VDPAKRITIA QIRQHRWMQA DPTLLQQDDP AFSMQGYTSN LGDYNEQVLG IMQALGIDRQ RTVESLQNSS YNHFAAIYYL LLERLREHRS TQPSSRATPA PARQPQLRNS DLSSLEVPQE ILPCDPFRPS LLCPQPQALA QSVLQAEIDC DLHSSLQPLF FPLDTNCSGV FRHRSISPSS LLDTAISEEA RQGPSLEEEQ EVQEPLPGST GRRHTLAEVS THFSPLNPPC IIVSSSAAVS PSEGTSSDSC LPFSASEGPA GLGGGLATPG LLGTSSPVRL ASPFLGSQSA TPVLQSQAGL GATVLPPVSF QEGRRASDTS LTQGLKAFRQ QLRKNARTKG FLGLNKIKGL ARQVCQSSIR GSRGGMSTFH TPAPSSGLQG CTASSREGRS LLEEVLHQQR LLQLQHHSAV SSDYQQAPQL SPVPYVLTPC DGLLVSGIPL LPTPLLQPGM SPVASAAQLL DAHLHISAGP VALPTGPLPQ CLTRLSPSCD PAGLPQGDCE MEDLTSGQRG TFVLVQ

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, CRTC1/TORC1 and CRTC2/TORC2. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators CRTC1/TORC1 and CRTC2/TORC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. In concert with CRTC1/TORC1, regulates the light-induced entrainment of the circadian clock by attenuating PER1 induction; represses CREB-mediated transcription of PER1 by phosphorylating and deactivating CRTC1/TORC1.

Gene References into Functions

Results indicate that overexpressing SIK1 can phosphorylate CRTC1 and CRTC2 in the norepinephrine (NE)-stimulated pinealocytes. Howeve, the endogenous SIK1 does not appear to be the main regulator of their phosphorylation and intracellular localization. PMID: 26210066
Results show a role of SIK1 in gastrin induced responses and suggest that SIK1 may act as tumour suppressor in gastric adenocarcinoma cells. PMID: 25384047
SIK1 serves as a part of a self-regulatory circuit to modulate insulin secretion from pancreatic beta-cells by controlling cAMP concentration through modulation of PDE4D activity. PMID: 25918234
these data identify a previously unrecognized mechanism by which SIK1 mediates the activation of MEF2-dependent transcription by BDNF. PMID: 23349925
acute cocaine treatment of rats induced the expression of MEF2C in the striatum via activation of SIK1 and the phosphorylation of HDAC5 PMID: 21954104
SIK2 mediates TORC2 inactivation in basal conditions, whereas induction of SIK1 limits transcriptional activation in the hypothalamic paraventricular nucleus PMID: 22109884
These results provide evidence that SIK1 is present in lung epithelial cells and that its function is relevant for the action of isoproterenol during regulation of active sodium transport. PMID: 21549091
Nucleocytoplasmic shuttling of SIK may play important role in transcriptional regulation of cAMP-responsive element (CRE)-dependent gene expression. PMID: 12530631
SIK isoforms regulate hormonal signal transduction in both adrenal and adipose tissues. PMID: 15134808
Salt-inducible kinase-1 represses cAMP response element-binding protein activity both in the nucleus and in the cytoplasm PMID: 15511237
Ser186 in the activation loop is the site of autophosphorylation and essential for the kinase activity. PMID: 18348280
Results show that the salt-inducible kinase family of proteins regulates hepatic lipogenesis by modulating SREBP-1c activity. PMID: 19244231
Our cumulative results support SIK1 having a repressor role on the NE-induced Aa-nat expression and that this repression by SIK1 may play an important role in determining the duration of the arylalkylamine N-acetyltransferase response. PMID: 19470703
A distinct intracellular signaling network, with SIK1 at its core, which is triggered by a monovalent cation (Na(+), links sodium permeability to its active transport. PMID: 17939993
Blocking the SIK1 network using negative mutants as well as different stages of its activation pathway prevented the effects induced by the hypertensive alpha-adducin. PMID: 19657284
Snf1lk expression is controlled by a cyclic AMP mechanism. PMID: 19103603

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Subcellular Location

Cytoplasm. Nucleus. Note=Following ACTH (adrenocorticotropic hormone) treatment and subsequent phosphorylation by PKA, translocates to the cytoplasm, where it binds to YWHAZ.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, AMPK subfamily

Database Links

KEGG: rno:59329

STRING: 10116.ENSRNOP00000001579

UniGene: Rn.42905

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Recombinant Rat Serine/threonine-protein kinase SIK1 (Sik1)

Product Details

Customer Reviews and Q&A

Target Background

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