Recombinant Rat Sodium/hydrogen exchanger 1(Slc9a1) ,partial

Code CSB-YP021726RA
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Source Yeast
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Code CSB-EP021726RA
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Source E.coli
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Code CSB-EP021726RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021726RA
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Source Baculovirus
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Code CSB-MP021726RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Slc9a1
Uniprot No. P26431
Alternative Names Slc9a1; Nhe1; Sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; NHE-1; Solute carrier family 9 member 1
Species Rattus norvegicus (Rat)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Gene References into Functions
  1. Endurance training increased NHE1 and NBC1 genes and proteins expression in both Extensor digitorum longus and Soleus in the diabetic training group compared to control groups. PMID: 28719339
  2. Buffer-induced alkalemia after ROSC worsened myocardial function and survival, likely through enhancing NHE-1 activity. PMID: 27633736
  3. The results demonstrate that in RGM1 cells, migratory velocity is increased by NHE1 activation, while NHE2 activity inhibit this process. PMID: 28019659
  4. miR-185 directly targets the 3'-untranslated region of Na+/H+ exchanger-1 (NHE-1), a protein involved in endoplasmic reticulum stress. PMID: 26521941
  5. During hypoxia, activation of ROCK enhances NHE1 activity and promotes pulmonary artery smooth muscle cell migration and proliferation. PMID: 27009277
  6. Data show that the cardioprotective effects of sevoflurane are mediated through an increase in nitric oxide synthase (NOS) and a decrease in phopshorylated Na+/H+ exchanger 1 (NHE1) levels. PMID: 26459736
  7. These results suggest that the stressful short-period and slow-paced treadmill exercise suppressed NHE1 and AQP4 expression resulting in the amelioration of brain edema at least partly via the moderate increase in plasma corticosterone levels. PMID: 26724742
  8. Enhanced expression of NHE-1, 2 and 4 proteins and mRNAs expression in cervix under genistein influence could help to restore the cervicovaginal fluid pH that might help to prevent cervicovaginal complications related to menopause PMID: 26078707
  9. NHE1 mediated hypertrophy is accounted for by increased activation and phosphorylation of p90 ribosomal S6 Kinase. PMID: 25830299
  10. Animals treateanimals treated with L-shNHE1 exhibited a reduced LV wall thickness. PMID: 25749446
  11. Mutagenesis analyses identified residues in transmembrane helix M9 and exofacial re-entrant loop EL5 of NHE1 that affect substrate affinities and/or drug sensitivity. PMID: 26063808
  12. AKT, through an inhibitory phosphorylation of the NHE-1, prevents its stretch-induced activation. PMID: 25240639
  13. Early and transient NHE-1 inhibition was found to prevent hypertrophy and heart failure. PMID: 25216745
  14. activates autophagy via Akt pathway and reduces endoplasmic reticulum stress-induced injury in podocytes PMID: 24566932
  15. Disruption of the DNM2-dependent retrograde trafficking of NHE1 contributes to cardiomyocyte apoptosis. PMID: 23837875
  16. MIneralcorticoid receptor antagonism may be followed by NHE1 activation which is deleterious to the heart. PMID: 24126173
  17. It was concluded that strenuous endurance exercise increases myocardial NHE1 activity at physiological pH, which would likely enhance cardiac performance under physiological conditions. PMID: 24049114
  18. Acid loading induced glomerular mesangial cell proliferation through NHE1-dependent pathways; our findings may contribute to the understanding of metabolic acidosis in the progression of chronic kidney disease PMID: 23545808
  19. Differential expressions of uterine NHE isoforms 1, 2, and 4 could explain the observed changes in the uterine fluid pH under sex steroid effect and at different phases of the oestrous cycle PMID: 23509787
  20. data indicate that exercise training at an intensity that results in cardiac hypertrophy and improved performance is accompanied by decreased NHE1 content in heart. PMID: 23055051
  21. CAII/NHE1 interaction constitutes a component of the slow force response to heart muscle stretch, which potentiates NHE1-mediated H(+) transport in the myocardium. PMID: 23709596
  22. NHE1 plays a role in pain processing at peripheral and spinal levels. PMID: 22898152
  23. NHE-1 hammerhead ribozyme reduces expression of NHE-1 mRNA, induces intracellular acidosis and consequently prohibits the proliferation of pulmonary artery smooth muscle cells. PMID: 21207853
  24. regulation of NHE1 activity through a direct protein-protein interaction that can be modulated by intracellular pH and Na(+) and Ca(2+) concentrations PMID: 22731252
  25. NHE1 directly binds to calcineurin A and activates downstream NFAT signaling, leading to cardiomyocyte hypertrophy PMID: 22688515
  26. This study evaluated the role for NHE-1 in diabetic cataract formation and retinal oxidative stress and apoptosis. PMID: 22407349
  27. investigation of rapid hormonal action/interaction in acid-base equilibrium in renal proximal straight tubule: Data suggest ALDO plays role in regulating pHi recovery after acid load induced by NH4Cl; effect mediated via basolateral NHE1 exchanger. PMID: 22154810
  28. Propose NHE-1 activation as key factor in determining the slow force response to stretch. PMID: 21659487
  29. NHE-1 participates in isoproterenol-induced downregulation of SERCA2a and development of cardiac remodeling in rat hearts. PMID: 21856903
  30. The regulatory interaction whereby NHE1 enhances the activity of NHE3 in the medullary thick ascending limb plays a role in the chronic regulation of HCO(3)(-) absorption. PMID: 21613418
  31. The findings suggest that NHE-1-mediated disruption of ionic homeostasis contributes to striatal and CA1 pyramidal neuronal injury after neonatal hypoxia/ischemia. PMID: 20712402
  32. Report altered expression of Na(+)-K(+) pump subunits, FXYD1 and NHE1 depending on training status, intensity and muscle type. PMID: 21325644
  33. cardiac hypertrophy linked with mitochondrial fission/fusion protein expression affected by NHE-1 inhibition PMID: 20886221
  34. studies on protein translocation in cardiomyocytes in response to stimuli: translocation of NHE1 & GLUT4 appears linked, provide spatially/temporally co-ordinated responses to insulin or challenges in glucose metabolism & cell pH PMID: 20868366
  35. Results demonstrate that increased NHE1 protein expression makes cells more susceptible to damage induced by hypoxia/reoxygenation in isolated cardiomyocytes. PMID: 19949839
  36. thyroid hormone and thyroid hormone receptor TRalpha regulate expression of the Na+/H+ exchanger PMID: 12039959
  37. Altered expression of Na(+)/H(+) exchanger isoforms 1 and 3 in clipped and unclipped kidneys of a 2-kidney-1-clip Goldblatt model of hypertension. PMID: 12218313
  38. conserved motifs within intracellular domains 2 and 3 of selective subtypes of several G protein-coupled receptor families confer coupling to NHE1 PMID: 12566440
  39. stimulation by sustained intracellular acidosis PMID: 12791686
  40. mineralocorticoid/salt-induced cardiac fibrosis may involve coronary vascular smooth muscle cell NHE-1 activity as a contributor to transcriptional events that produce coronary vasculitis seen with excess mineralocorticoid and salt. PMID: 12933657
  41. NHE-1 as a new regulator of ERK activity in rat aortic vascular smooth muscle cells. PMID: 14600156
  42. The hypertrophic effect of aldosterone on ventricular myocytes is dependent on NHE-1 activity. PMID: 14610099
  43. While cAMP enhances taste receptor cells apical H(+) entry and chorda tympani responses to strong acid, an increase in Ca(2+) activates NHE-1 and increases neural adaptation to all acidic stimuli. PMID: 14724181
  44. NHE-1 may be involved in the regulation of several vasoactive modulators that contribute to functional alterations in diabetic retinal microangiopathy. PMID: 14737747
  45. Activation of Na+/H+ exchanger system may enhance progress of cerebral damage and oedema following glutamate exposure. PMID: 14753440
  46. in a neonatal model, the development of an acidic surface pH can be ascribed, in part, to progressive stratum corneum acidification by two endogenous mechanisms, namely, sPLA2 and NHE1 PMID: 15009712
  47. NHE-1 in the nuclear membrane may play a role in the modulation of intranuclear pH. PMID: 15523538
  48. basolateral NHE1 regulates apical NHE3 and HCO3- absorption in the MTAL by controlling the organization of the actin cytoskeleton. PMID: 15644322
  49. Data show that the Na+/H+ exchanger (NHE), NHE1, is functionally active in HEK 293 cells, and resides in lipid rafts or caveolae, which may create cellular microdomains where pH(i) is tightly regulated. PMID: 16002403
  50. In conclusion, EGF protects esophageal epithelial cells against acid through NHE activation via Ca(2+)/calmodulin and the PKC pathway. PMID: 16306134

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Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Protein Families Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
Tissue Specificity Not tissue specific.
Database Links

KEGG: rno:24782

STRING: 10116.ENSRNOP00000011049

UniGene: Rn.5025

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