Recombinant Rat Speedy protein A (Spdya)

Code CSB-YP837145RA
MSDS
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Source Yeast
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Code CSB-EP837145RA
MSDS
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Source E.coli
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Code CSB-EP837145RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837145RA
MSDS
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Source Baculovirus
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Code CSB-MP837145RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Spdya
Uniprot No.
Alternative Names
Spdya; Spdy1; Speedy protein A; GS4; Rapid inducer of G2/M progression in oocytes A; RINGO A; Speedy-1; Spy1
Species
Rattus norvegicus (Rat)
Expression Region
1-312
Target Protein Sequence
MRHNQMCCET PPTVTVHVKS GSNRSHQTRK PVSLKRPILK DSWQASEKNA HNNKPKRPRG PCLIIQRQEM TAFFKLFDDD LIQDFLWMDC CCKIADKYLL AMTFVYFKRA KFTISEHTRI NFFIALYLAN TVEEDEEEAK YEIFPWALGK NWRKLFPNFL KLRDQLWDRI DYRAIVSRRC CEEVMAIAPS HYIWQRERSV HHSGAARNYN RDEVHLPRGP SATPVDCSLC GKKGRYVRLG LSSSSSSSSD IVELTGKRSQ ELHNSLSMDM IGDPSQANTY SQVANDHQSK KENETNFVKK TKSMGWFAES EE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2. Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Interferes with CDKN1B-mediated inhibition of CDK2. Mediates cell survival during the DNA damage process through activation of CDK2.
Gene References into Functions
  1. LM23 played a protective role in H2O2-induced apoptosis of 293T cells, mediated at least in part through the Akt/PI3K signal pathway; these results provide new insights into the role of LM23 in the development of the testes and spermatogenesis. PMID: 23685908
  2. Spy1 participated in the pathological process response to sciatic nerve injury and may be associated with Schwann cells proliferation and axons regeneration. PMID: 23129232
  3. In the hippocampus, SpeedyRINGO acts as a savior for neurons that are under apoptosis. PMID: 22751172
  4. Peripheral nerve injury induced an up-regulation of Spy1 in lumbar spinal cord, which was associated with glial proliferation. PMID: 19082704
  5. testis-specific gene LM23 identified & characterized from rat type A spermatogonia, pachytene spermatocytes, & round spermatids. PMID: 17376406
  6. LM23 is essential for spermatogenesis in Rattus norvegicus. PMID: 20036869

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Subcellular Location
Nucleus.
Protein Families
Speedy/Ringo family
Database Links
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