Recombinant Rat Sulfotransferase family cytosolic 2B member 1 (Sult2b1)

Code CSB-YP644962RA
MSDS
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Source Yeast
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Code CSB-EP644962RA
MSDS
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Source E.coli
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Code CSB-EP644962RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP644962RA
MSDS
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Source Baculovirus
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Code CSB-MP644962RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sult2b1
Uniprot No.
Alternative Names
Sult2b1; Sulfotransferase 2B1; EC 2.8.2.2; Alcohol sulfotransferase; Hydroxysteroid sulfotransferase 2; Sulfotransferase family cytosolic 2B member 1; ST2B1
Species
Rattus norvegicus (Rat)
Expression Region
1-340
Target Protein Sequence
MDGPQPPALW GSLENRVSEL SQKLQGEYFR YKGIPFPVGM YTPESLSLAE NTSNVRDDDI FIVTYPKSGT NWMIEIICLI LKDGDPSWIR SEPIWQRAPW CETTISAFSL PERPSPRLMC SHLPIELFTK AAFSSKAKVI YLGRNPRDVV VSLYYYSKIA VQLKDPGTPE QFLQNFLKGE VQFGSWFDHI KGWIRMRGRE NFLFITYEEL QQDLRGSVQL ICEFLGRPLG EEALSSVVAH SAFAAMKANN MSNYTLLPAS LLDHRQGAFL RKGISGDWKN HFTVAQSETF DQVYREQMHG LPSFPWDRSA EDGSPDGETE PSPSPSPGLA SDDPNPGSSQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfonates cholesterol. Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA). Conjugates efficiently cholesterol but has a greater affinity for pregnenolone sulfation. Does not show high activity with DHEA. Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation.; Prefers pregnenolone over DHEA as a substrate and does not sulfate cholesterol.
Gene References into Functions
  1. Since 1'-hydroxyestragole exposure readily produced DNA injury in B-13/H cells, these data suggest that cholangiocarcinomas generated in rats fed estragole may be dependent, in part, on SULT2B1 activation of the 1'-hydroxyestragole metabolite. PMID: 26739637
Subcellular Location
Cytoplasm, cytosol. Microsome. Nucleus.
Protein Families
Sulfotransferase 1 family
Tissue Specificity
Isoform 1 is expressed in skin and testis. Higher level of isoform 2 expressed in skin and intestine, moderate level in the kidney, low level in liver, stomach and placenta.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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