Recombinant Rat Uromodulin (Umod)

Code CSB-YP025616RA
MSDS
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Source Yeast
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Code CSB-EP025616RA
MSDS
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Source E.coli
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Code CSB-EP025616RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025616RA
MSDS
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Source Baculovirus
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Code CSB-MP025616RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Umod
Uniprot No.
Alternative Names
UmodUromodulin; Tamm-Horsfall urinary glycoprotein; THP) [Cleaved into: Uromodulin; secreted form]
Species
Rattus norvegicus (Rat)
Expression Region
26-615
Target Protein Sequence
NDSPE ARRCSECHDN ATCVLDGVVT TCSCQAGFTG DGLVCEDIDE CATPWTHNCS NSICMNTLGS YECSCQDGFR LTPGLGCIDV NECTEQGLSN CHSLATCVNT EGSYSCVCPK GYRGDGWYCE CSPGFCEPGL DCLPQGPSGK LVCQDPCNVY ETLTEYWRST DYGAGYSCDS DMHGWYRFTG QGGVRMAETC VPVLRCNTAA PMWLNGSHPS SREGIVSRTA CAHWSDHCCL WSTEIQVKAC PGGFYVYNLT EPPECNLAYC TDPSSVEGTC EECGVDEDCV SDNGRWRCQC KQDFNVTDVS LLEHRLECEA NEIKISLSKC QLQSLGFMKV FMYLNDRQCS GFSERGERDW MSIVTPARDG PCGTVLRRNE THATYSNTLY LASEIIIRDI NIRINFECSY PLDMKVSLKT SLQPMVSALN ISLGGTGKFT VQMALFQNPT YTQPYQGPSV MLSTEAFLYV GTMLDGGDLS RFVLLMTNCY ATPSSNSTDP VKYFIIQDRC PHTEDTTIQV TENGESSQAR FSIQMFRFAG NSDLVYLHCE VYLCDTMSEQ CKPTCSGTRY RSGNFIDQTR VLNLGPITRQ GVQAS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.; In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals.
Gene References into Functions
  1. Two forms of uromodulin (polymerization competent and polymerization incompetent) were found in urine from both Wistar-Kyoto and SHRSP, where the polymerization-incompetent form was increased in a pregnancy-specific manner in stroke-prone spontaneously hypertensive rats. PMID: 28348009
  2. results demonstrate a selective decrease in medullary THP synthesis, suggesting a potential involvement of THP in the renal functional changes associated with hypothyroidism PMID: 15007654
  3. Tamm-Horsfall protein derived from hyperoxaluric rats exhibited 2-fold increase in the Ca*Ox binding when compared to controls PMID: 15992786
  4. Sepsis-induced renal Cox-2 expression on TLR4 is tubule specific. The TLR4-dependent Cox-2 expression is mostly restricted to cortical and medullary thick ascending loops of Henle that characteristically express and secrete Tamm-Horsfall protein. PMID: 17634395
  5. The finding that LZP might act as a new partner of THP would provide novel insights into renal functions related to THP and LZP, such as the urothelial permeability barrier and the host defense against the adhesion of pathogens. PMID: 18830570

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Subcellular Location
Apical cell membrane; Lipid-anchor, GPI-anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane.; [Uromodulin, secreted form]: Secreted.
Tissue Specificity
Expression restricted to the thick ascending limb of the loop of Henle (TALH).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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