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Recombinant Saccharomyces cerevisiae Cell division control protein 6 (CDC6), partial

Recombinant Saccharomyces cerevisiae Cell division control protein 6 (CDC6), partial

Code	CSB-YP362593SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP362593SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP362593SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP362593SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP362593SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CDC6

Uniprot No.

P09119

Alternative Names

CDC6; YJL194W; J0347Cell division control protein 6

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.

Gene References into Functions

Cdc6 degradation triggered by Mck1 kinase was enhanced upon DNA damage. PMID: 25995377
Cdc6 ATP hydrolysis is therefore required for Cdc6 disengagement from the pre-replicative complex after helicase loading to advance subsequent steps in helicase activation in vivo. PMID: 26305410
We propose that both CDK and Mck1 kinases are required for Cdc6 degradation to ensure a tight control of DNA replication. PMID: 23236290
the mitotic CDK, Clb2/Cdc28, binds tightly to an amino-terminal domain of Cdc6, and Cdc6 in this complex is unable to assemble pre-replicative complexes PMID: 15496876
Cdc6 and origin DNA activate a molecular switch in Origin Recognition Complex that contributes to pre-RC assembly. PMID: 16228006
findings show that Mcm2-7 loading requires the coordinate action of the origin recognition complex and Cdc6 ATPases PMID: 16387651
Cdc6 protein is crucial for DNA replication PMID: 17267692
Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication PMID: 17314092