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Recombinant Saccharomyces cerevisiae Elongation factor 1 alpha-like protein (HBS1), partial

Recombinant Saccharomyces cerevisiae Elongation factor 1 alpha-like protein (HBS1), partial

Code	CSB-YP333731SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP333731SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP333731SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP333731SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP333731SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

HBS1

Uniprot No.

P32769

Alternative Names

HBS1; YKR084C; YKR404; Elongation factor 1 alpha-like protein

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs.

Gene References into Functions

analysis establishes the structural foundation for the recognition of aberrantly stalled 80S ribosomes by the Dom34.Hbs1.GTP complex during Dom34-mediated mRNA surveillance pathways PMID: 27995908
Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx. PMID: 22981232
Dom34:Hbs1 facilitates the decay of nonstop mRNAs from the 3' end by exosomes and is required for the complete degradation of nonstop mRNA decay intermediates. PMID: 22503425
The close structural similarity of Dom34 and Hbs1 to eukaryotic release factors (eRFs) enabled the authors to propose a model for the ribosome-bound eRF1-eRF3 complex. PMID: 21623367
findings show that Dom34:Hbs1 (two interacting factors genetically implicated in no-go decay) interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off PMID: 20947765
Dom34 has a role in an mRNA decay pathway called No-Go decay and the interaction of Dom34 with Hbs1 results in an increase in the affinity constant of Hbs1 for GTP but not GDP PMID: 18180287