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Recombinant Saccharomyces cerevisiae DNA polymerase epsilon catalytic subunit A (POL2), partial

Recombinant Saccharomyces cerevisiae DNA polymerase epsilon catalytic subunit A (POL2), partial

Code	CSB-YP018309SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP018309SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP018309SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP018309SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP018309SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

POL2

Uniprot No.

P21951

Alternative Names

POL2; DUN2; YNL262W; N0825DNA polymerase epsilon catalytic subunit A; EC 2.7.7.7; 3'-5' exodeoxyribonuclease; EC 3.1.11.-; DNA polymerase II subunit A

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Catalytic component of the DNA polymerase epsilon complex which participates in chromosomal DNA replication. Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork. Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication.

Gene References into Functions

To dissect the Saccharomyces cerevisiae RNA polymerase II TL at individual-residue resolution, we quantitatively phenotyped nearly all TL single variants en masse.Our structural genetics approach incorporates structural and phenotypic data for high-resolution dissection of transcription mechanisms and their evolution, and is readily applicable to other essential yeast proteins. PMID: 27898685
dNTP pool levels correlate with Pol epsilon mutator severity PMID: 25827226
Authors report the presence of a [Fe-S] cluster directly within the active polymerase domain of Pol2 (residues 1-1187). PMID: 24144619
Labeling of DNA polymerase epsilon catalytic subunit with the nick-containing DNA repair intermediate indicates that the DNA polymerase is involved in DNA repair in yeast PMID: 21601535
show that subunits of DNA polymerase epsilon (Pol epsilon) are required but that Mrc1, a polymerase epsilon-associated checkpoint protein, is opposing senescence PMID: 21321081
The active site location occupied by Met644 in Pol epsilon is a key determinant of DNA replication fidelity. PMID: 17452367
RNAPII elongation is stimulated by H2B-K123 ubiquitination independent of H3-K4 methy PMID: 17543890
findings reveal a pattern indicating that DNA polymerase epsilon participates in leading-strand DNA replication PMID: 17615360
a mutation in the 3'-5' exonuclease domain of Pol2 dramatically reduces the frequency of end joins formed with initial 3' flaps. Thus, Pol2 performs a key 3' end-processing step in NHEJ. PMID: 18437220