Recombinant Salmonella typhimurium Periplasmic serine endoprotease DegP (degP)

Code CSB-YP328669SXB
MSDS
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Source Yeast
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Code CSB-EP328669SXB
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Source E.coli
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Code CSB-EP328669SXB-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328669SXB
MSDS
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Source Baculovirus
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Code CSB-MP328669SXB
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
degP
Uniprot No.
Alternative Names
degP; htrA; ptd; STM0209Periplasmic serine endoprotease DegP; EC 3.4.21.107; Heat shock protein DegP; Protease Do
Species
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Expression Region
27-475
Target Protein Sequence
AETS SSAMTAQQMP SLAPMLEKVM PSVVSINVEG STTVNTPRMP RNFQQFFGDD SPFCQDGSPF QNSPFCQGGG NGGNGGQQQK FMALGSGVII DAAKGYVVTN NHVVDNASVI KVQLSDGRKF DAKVVGKDPR SDIALIQIQN PKNLTAIKLA DSDALRVGDY TVAIGNPFGL GETVTSGIVS ALGRSGLNVE NYENFIQTDA AINRGNSGGA LVNLNGELIG INTAILAPDG GNIGIGFAIP SNMVKNLTSQ MVEYGQVKRG ELGIMGTELN SELAKAMKVD AQRGAFVSQV MPNSSAAKAG IKAGDVITSL NGKPISSFAA LRAQVGTMPV GSKISLGLLR EGKAITVNLE LQQSSQSQVD SSTIFSGIEG AEMSNKGQDK GVVVSSVKAN SPAAQIGLKK GDVIIGANQQ PVKNIAELRK ILDSKPSVLA LNIQRGDSSI YLLMQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures. It degrades transiently denatured and unfolded proteins which accumulate in the periplasm following heat shock or other stress conditions. DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated temperatures. It can degrade IciA, ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP).
Gene References into Functions
  1. Both the protease and chaperone/sequestering functions of HtrA operate in vivo during infection. PMID: 19246758
Subcellular Location
Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Peptidase S1C family
Database Links

KEGG: stm:STM0209

STRING: 99287.STM0209

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