Recombinant Sheep Pituitary-specific positive transcription factor 1 (POU1F1)

Code CSB-YP018391SH
MSDS
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Source Yeast
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Code CSB-EP018391SH
MSDS
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Source E.coli
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Code CSB-EP018391SH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018391SH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP018391SH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
POU1F1
Uniprot No.
Alternative Names
POU1F1; PIT-1; PIT1; Pituitary-specific positive transcription factor 1; PIT-1; Growth hormone factor 1; GHF-1
Species
Ovis aries (Sheep)
Expression Region
1-291
Target Protein Sequence
MSCQPFTSTD TFIPLNSESS ATLPLIMHPS AAECLPVSNH ATNVMSTATG LHYSVPSCHY GNQSSTYGVM AGSLTPCLYE FPDHTLSHGF PPMHQPLLSE DPTAADFKQE LRRKSKLIEE PIDMDSPEIR ELEKFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL FKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS SQEILRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FPNSKEHLEC R
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
Gene References into Functions
  1. Weaning weight of Lori-Bakhtiari and Zel sheep breeds were affected by polymorphism in exon 3 of POU1F1 gene, suggesting that this polymorphism can be used as a molecular marker for this trait. PMID: 24965143
  2. it can be assumed that exon 3 POU1F1 in these Iranian indigenous sheep breeds has high variability and the SNP observed in this study are associated with weaning weight trait PMID: 25572243
  3. Molecular cloning, sequencing and characterization of POU1F1 PMID: 16636924
  4. isolated three additional splicing variants: POU1F1-beta, with a 78 bp insert in the trans-activation domain; POU1F1-gamma that lacks exon 3 and POU1F1-delta that lacks exons 3, 4 and 5 PMID: 16942842
Subcellular Location
Nucleus.
Protein Families
POU transcription factor family, Class-1 subfamily
Database Links

KEGG: oas:443370

UniGene: Oar.781

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