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Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1), partial

Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1), partial

Code	CSB-YP323400TGA
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP323400TGA
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP323400TGA-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP323400TGA
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP323400TGA
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ORF1

Uniprot No.

P15962

Alternative Names

ORF1; RNA-directed RNA polymerase; EC 2.7.7.48; Protein p92) [Cleaved into: Protein p33]

Species

Tomato bushy stunt virus (strain Cherry) (TBSV)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

RNA-dependent RNA polymerase that plays an essential role in the virus replication.

Gene References into Functions

Conserved motifs in polymerase(p92) modulate genome replication, subgenomic transcription, and amplification of defective interfering RNAs. PMID: 25568204
analysis of p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract PMID: 22933278
Deletion of Ino2 and Ino4 in S. cerevisiae reduces TBSV RNA accumulation and inhibits the p33 and p92 activity. PMID: 21561636
p33 interacts with p92 in vitro and that the interaction requires the S1 subdomain, whereas the S2 subdomain plays lesser function. PMID: 16242746
An in vitro assay with the tombusvirus replicase indicated that Rpb11p affects TBSV replication and recombination only indirectly, via regulating p33 and p92(pol) levels. PMID: 17689583
The in vitro assembly of the functional tombusvirus replicase required rATP and rGTP and the membranous fraction and Ssa1p (Hsp70). PMID: 19060219