Recombinant Xenopus laevis Doublesex- and mab-3-related transcription factor 1A (dmrt1-a)

Code CSB-YP665993XBE
MSDS
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Source Yeast
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Code CSB-EP665993XBE
MSDS
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Source E.coli
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Code CSB-EP665993XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP665993XBE
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP665993XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dmrt1-a
Uniprot No.
Alternative Names
dmrt1-a; dmrt1Doublesex- and mab-3-related transcription factor 1A; xDmrt1; DMRT1-alpha
Species
Xenopus laevis (African clawed frog)
Expression Region
1-336
Target Protein Sequence
MQNNEEPYSK TRNSGQHPSG VHTKKSPRLP KCARCRNHGY ASPLKGHKRY CMWRDCQCKK CSLIAERQRV MAAQVALRRQ QAQEEELGIS HPIHLPIAAE LLIKKEHGGS SSCLMLENSS TQTTSTPTSG STASSEGKVL IQEIPSITSR GHMESTSDLV MDSPYYSNFY QPPLYPYYNN LYNYPPYQMA MAAESTSGND MGGISGPPLK NNHRNHPAAY VPSQSGNQWQ MKNRENRFPG HSGSSQFRMH SYYPPYLGQS VPNPACVPPF LTFEEIPSYS EAKASVLSPP SSQDSGVISL SSNSPVSNES TKAVAEQEPN SESSLFTVTT AAENGE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and germ cell proliferation. Acts both as a transcription repressor and activator.
Gene References into Functions
  1. DMRT1 and its W-linked paralogue, DM-W, have opposite roles in sexual dimorphism of Xenopus laevis PMID: 20573695
Subcellular Location
Nucleus.
Protein Families
DMRT family
Tissue Specificity
Expressed throughout the embryo during early development, becoming restricted to the primordial gonads in stage 52 tadpoles. Expressed exclusively in the primordial gonads of both ZW and ZZ tadpoles. Specifically expressed in the somatic cells surrounding
Database Links

KEGG: xla:735040

UniGene: Xl.50728

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