Recombinant Xenopus laevis Iroquois-class homeodomain protein irx-3 (irx3)

Code CSB-YP011835XBE
MSDS
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Source Yeast
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Code CSB-EP011835XBE
MSDS
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Source E.coli
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Code CSB-EP011835XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011835XBE
MSDS
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Source Baculovirus
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Code CSB-MP011835XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
irx3
Uniprot No.
Alternative Names
irx3; iro3Iroquois-class homeodomain protein irx-3; Iroquois homeobox protein 3; Xiro3
Species
Xenopus laevis (African clawed frog)
Expression Region
1-448
Target Protein Sequence
MSFPQLGYQY IRPLYPSDRQ SVGVTRSGTE LSPAGTLSNV LSSVYGAPYA AAAAAQAYGA FLPYSAELPI FPQLGSQYDM KDSPGVQHAA FSHPHPAFYP YGQYQFGDPS RPKNATREST STLKAWLNEH RKNPYPTKGE KIMLAIITKM TLTQVSTWFA NARRRLKKEN KMTWAPRSRT DEEGNAYGSD HEEDKHEDEE EIDLENIDTE DIESKEDLDD PDTDIHSDSK TDTRSDSEVS DGFEDLNVPE DRLLKSVVGQ RQLLNEEPQD KCALSSDAKV PQPACEQIKL NRLPPSPPQE NNMPVAHKPK IWSLAETATT PDNPRNSPNT GGSVNTQNLI AQHRLIASPG SRFQGWTGRA FSAQQLSLLN SAHFLQGLGV SHTAPCSGNA SFPKAAESKC STNSLTDRPS TVDIEKTIPV LNTAFQPVQR RSQNHLDAAM ILSALSSS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts partially redundantly with other irx members in neural patterning. Required for formation of the posterior forebrain, midbrain, hindbrain, and to a lesser extent, spinal cord. Both up-regulates and down-regulates gene expression during neural development. Acts early in neural plate development to induce proneural gene expression and specify a neural precursor state. Also up-regulates repressors that prevent neuronal differentiation. Required during at least two stages of pronephros kidney development; during neurula stages, maintains transcription of key renal genes to define the size and identity of the pronephric anlage, probably in part through regulation of bmp-signaling. Subsequently required for proper formation of the intermediate tubule segment of the pronephros.
Gene References into Functions
  1. Irx3 is transcriptionally induced by the Wnt/beta-catenin signaling pathway during anteroposterior patterning of the neural axis. PMID: 20979027
  2. Irx3 is the first gene known to be necessary and sufficient to specify nephron segment fate in vivo. PMID: 17875669
Subcellular Location
Nucleus.
Protein Families
TALE/IRO homeobox family
Tissue Specificity
Primarily expressed in the developing central nervous system (CNS). At gastrula stage, expressed in both the superficial and deep layers of the presumptive neural plate with expression spreading to the prospective hindbrain, spinal cord and midbrain-hindb
Database Links

KEGG: xla:399365

UniGene: Xl.4522

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