Recombinant Xenopus laevis LIM/homeobox protein Lhx1 (lhx1)

Code CSB-YP012919XBE
MSDS
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Source Yeast
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Code CSB-EP012919XBE
MSDS
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Source E.coli
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Code CSB-EP012919XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012919XBE
MSDS
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Source Baculovirus
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Code CSB-MP012919XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lhx1
Uniprot No.
Alternative Names
lhx1; lim-1; lim1; LIM/homeobox protein Lhx1; LIM homeobox protein 1; Homeobox protein Lim-1; Xlim1; x-Lhx1; xLIM-1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-403
Target Protein Sequence
MVHCAGCERP ILDRFLLNVL DRAWHVKCVQ CCECKCNLTE KCFSREGKLY CKNDFFRRFG TKCAGCAQGI SPSDLVRRAR SKVFHLNCFT CMMCNKQLST GEELYIIDEN KFVCKEDYLN NNNAAKENSF ISVTGSDPSL SPESQDPLQD DAKDSESANV SDKEAGINEN DDQNLGAKRR GPRTTIKAKQ LETLKAAFAA TPKPTRHIRE QLAQETGLNM RVIQVWFQNR RSKERRMKQL SALGARRHAF FRSPRRMRPL VDRLEPGELI PNGPFAFYGD YQSEYYGPGS NYDFFPQGPP SSQAQTPVDL PFVPSSVPAG TPLGAMDHPI PGHHPSSDAQ RFTDIMSHPP GDSPSPEPNL PGSMHSMSAE VFGQSPPFSS LSVNGGYGNH LSHPPEMNET AVW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the establishment of the body plan via the Spemann organizer during gastrulation. Transcriptional activator required to induce organizer gene expression downstream of siamois. Promotes head formation by binding to 5'-TAAT'-3' elements in the promoters of head organizer genes cer1 and gsc to stimulate expression. Binds as a complex with siamois and mix-A/mix.1 to the cer1 promoter, and with ldb1 and otx2 to the gsc promoter. Also involved in neural induction via the organizer, including a role in notochord formation. Acts synergistically with ldb1 and ssbp in subsequent axis formation. Involved in kidney development, acting synergistically with pax8 to establish the pronephric primordium in late gastrulae/early neurulae and with pax2 during pronephric morphogenesis in tailbud stages. Has a later role in mediating the activity of inhibitors of ventralization.
Gene References into Functions
  1. These results demonstrate an essential role for Lhx1 in driving specification of the entire kidney field from the intermediate mesoderm. PMID: 21526205
  2. Substantial evidence is found for a prosomeric organization of the diencephalon based on alternating expression of Lhx1/5 and Lhx2/9 genes during larval development and in the adult. PMID: 15024752
Subcellular Location
Nucleus.
Tissue Specificity
Exhibits a biphasic expression pattern. Initially localized to the Spemann organizer region of gastrulae, leading to expression in prechordal mesoderm and notochord. In the second phase, expressed in the lateral mesoderm and neural plate, eventually conce
Database Links

KEGG: xla:399323

UniGene: Xl.32655

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