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Recombinant Xenopus laevis Transforming acidic coiled-coil-containing protein 3 (tacc3), partial

Recombinant Xenopus laevis Transforming acidic coiled-coil-containing protein 3 (tacc3), partial

Code	CSB-YP868695XBE
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP868695XBE
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP868695XBE-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP868695XBE
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP868695XBE
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

tacc3

Uniprot No.

Q9PTG8

Alternative Names

tacc3; maskin; Transforming acidic coiled-coil-containing protein 3; Cytoplasmic polyadenylation element-binding protein-associated factor Maskin; CPEB-associated factor Maskin

Species

Xenopus laevis (African clawed frog)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Maternal RNA in oocytes remains in a dormant state as masking outcompetes eif4g to bind eif4e, thereby preventing translation. During oocyte maturation this complex dissolves and eif4g binds eif4e to allow translation of maternal RNAs. Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in microtubule assembly during M-phase. Involved in spindle pole assembly by recruiting and regulating ckap5 at the spindle pole leading to microtubule elongation.

Gene References into Functions

TACC1 and TACC3 are each required for maintaining normal microtubule growth speed in Xenopus PMID: 26012630
XTACC3 can induce the recruitment of larger amounts of XMAP215 by increasing its local concentration, thereby promoting efficient microtubule elongation during mitosis PMID: 25262927
TACC3 promotes axon outgrowth and regulates microtubule dynamics in multiple embryonic cell types. PMID: 25187649
Maskin functions in mitotic spindle assembly. PMID: 15788567
Maskin activity and localization is controlled by differential phosphorylation PMID: 16107707
Maskin phosphorylation-dephosphorylation also oscillates with the cell cycle and is controlled by the kinase CDK1 and the phosphatase calcineurin PMID: 17086181
Maskin mRNA is translationally regulated by at least two repressor elements and an activation element. PMID: 17241108