Recombinant Xenopus tropicalis CUGBP Elav-like family member 1 (celf1)

Code CSB-YP630737XBF
MSDS
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Source Yeast
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Code CSB-EP630737XBF
MSDS
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Source E.coli
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Code CSB-EP630737XBF-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP630737XBF
MSDS
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Source Baculovirus
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Code CSB-MP630737XBF
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
celf1
Uniprot No.
Alternative Names
celf1; cugbp1; TEgg064f02.1CUGBP Elav-like family member 1; CELF-1; Bruno-like protein 2; CUG triplet repeat RNA-binding protein 1; CUG-BP1; CUG-BP- and ETR-3-like factor 1; RNA-binding protein BRUNOL-2
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region
1-490
Target Protein Sequence
MNGTMDHPDH PDPDSIKMFV GQVPRSWSEK ELRELFEQYG AVYEINVLRD RSQNPPQSKG CCFITFYTRK AALEAQNALH NMKVLPGMHH PIQMKPADSE KNNAVEDRKL FVGMVSKKCN ENDIRAMFSQ FGQIEESRIL RGPDGMSRGC AFVTFTTRSM AQMAIKAMHQ AQTMEGCSSP IVVKFADTQK DKEQKRMTQQ LQQQMQQLNA ASMWGNLAGL SSLAPQYLAL LQQTASSGNL NSLSGLHPMG GEYATGMTSG LNAMQLQNLA ALAAAASAAQ NTPSAGSALT TSSSPLSILT SSGSSPSSNN NSAVNPMASL GALQTLAGAT AGLNVGSLAG MAALNGGLGS SLSNGTGSTM EALSQAYSGI QQYAAAALPS LYNQSLLSQQ GLGAAGSQKE GPEGANLFIY HLPQEFGDQD LLQMFMPFGN VVSAKVFIDK QTNLSKCFGF VSYDNPVSAQ AAIQSMNGFQ IGMKRLKVQL KRSKNDSKPY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding protein implicated in the regulation of several post-transcriptional events. May be involved in pre-mRNA alternative splicing, mRNA translation activation and stability. Mediates the rapid and sequence-specific cytoplasmic deadenylation of EDEN-containing maternal mRNAs following fertilization. Binds to AU-rich sequences (AREs) of jun mRNA. Binds to the embryonic deadenylation element (EDEN) motif localized in the 3'-UTR of maternal mRNAs. Binds to RNA containing several repeats of the consensus sequence 5'-UGU-3'. EDEN-dependent deadenylation is enhanced by the presence of an additional cis element composed of three AUU repeats.
Gene References into Functions
  1. Identification of 153 mRNAs as new binding targets for EDEN-BP using microarrays. PMID: 18267972
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
CELF/BRUNOL family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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