Code | CSB-RA825959A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
The generation of the LMO2 recombinant monoclonal antibody involves a meticulously designed process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal, with the synthesized peptide derived from human LMO2 serving as the immunogen. Total RNA is extracted from these B cells and converted into cDNA through reverse transcription. The LMO2 antibody genes are then amplified using specific primers that target the antibody constant regions and are subsequently inserted into an expression vector. Through transfection, the vector is introduced into host cells, enabling the production of the LMO2 recombinant monoclonal antibody. Following cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography to obtain a highly purified form. The antibody's specificity and functionality are extensively characterized and validated through ELISA and WB analysis, ensuring its precise recognition of human LMO2 protein.
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